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Curr Opin Struct Biol. 2017 Oct;46:122-129. doi: 10.1016/j.sbi.2017.07.002. Epub 2017 Aug 5.

Atomic cryo-EM structures of viruses.

Author information

1
Department of Biological Sciences, Immunology and Infectious Disease, Purdue University, 240 S. Martin Jischke Drive, West Lafayette, IN 47907, USA; Department of Chemistry, Immunology and Infectious Disease, Purdue University, 240 S. Martin Jischke Drive, West Lafayette, IN 47907, USA; Markey Center for Structural Biology, Immunology and Infectious Disease, Purdue University, 240 S. Martin Jischke Drive, West Lafayette, IN 47907, USA; Purdue Institute of Inflammation, Immunology and Infectious Disease, Purdue University, 240 S. Martin Jischke Drive, West Lafayette, IN 47907, USA. Electronic address: jiang12@purdue.edu.
2
Department of Molecular Biosciences, University of Kansas, 1200 Sunnyside Avenue, Lawrence, KS 66045, USA. Electronic address: tangl@ku.edu.

Abstract

During the development of single particle cryo-EM in past five decades, icosahedral viruses have led the resolution progress owing to their large mass and high symmetry. Many technical advances in cryo-EM were first established with viruses. Since reaching ∼4Å resolution in 2008, it has become a relatively routine task to solve the atomic structure of isolated viruses. The future of structural virology will be increasingly focused on remaining challenges including solving structures of jumbo viruses, intermediate functional states during assembly, maturation, and infection, and in situ structures. Recent demonstrations of near-atomic resolution structure with electron tomography and sub-tomogram averaging opens a new direction for high resolution studies of pleomorphic viruses and the pleomorphic states of icosahedral viruses that have defied past efforts using the single particle cryo-EM approach.

PMID:
28787658
PMCID:
PMC5683926
DOI:
10.1016/j.sbi.2017.07.002
[Indexed for MEDLINE]
Free PMC Article

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