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Mol Microbiol. 2017 Oct;106(2):236-251. doi: 10.1111/mmi.13760. Epub 2017 Aug 22.

Carbonyl reduction by YmfI in Bacillus subtilis prevents accumulation of an inhibitory EF-P modification state.

Author information

1
Department of Biology, Indiana University, Bloomington, IN 47405, USA.
2
Department of Molecular Genetics and Center for RNA Biology, Ohio State University, Columbus, OH 43210, USA.
3
Department of Microbiology and Center for RNA Biology, Ohio State University, Columbus, OH 43210, USA.
4
Proteomics Facility, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109.

Abstract

Translation elongation factor P (EF-P) in Bacillus subtilis is required for a form of surface migration called swarming motility. Furthermore, B. subtilis EF-P is post-translationally modified with a 5-aminopentanol group but the pathway necessary for the synthesis and ligation of the modification is unknown. Here we determine that the protein YmfI catalyzes the reduction of EF-P-5 aminopentanone to EF-P-5 aminopentanol. In the absence of YmfI, accumulation of 5-aminopentanonated EF-P is inhibitory to swarming motility. Suppressor mutations that enhanced swarming in the absence of YmfI were found at two positions on EF-P, including one that changed the conserved modification site (Lys 32) and abolished post-translational modification. Thus, while modification of EF-P is thought to be essential for EF-P activity, here we show that in some cases it can be dispensable. YmfI is the first protein identified in the pathway leading to EF-P modification in B. subtilis, and B. subtilis encodes the first EF-P ortholog that retains function in the absence of modification.

PMID:
28787546
PMCID:
PMC5630506
DOI:
10.1111/mmi.13760
[Indexed for MEDLINE]
Free PMC Article

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