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Nat Rev Immunol. 2018 Jan;18(1):35-45. doi: 10.1038/nri.2017.76. Epub 2017 Aug 7.

Single-cell RNA sequencing to explore immune cell heterogeneity.

Author information

1
Center for Genomics and Systems Biology, New York University, New York, NY 10003-6688, USA.
2
New York Genome Center, New York, New York 10013, USA.

Abstract

Advances in single-cell RNA sequencing (scRNA-seq) have allowed for comprehensive analysis of the immune system. In this Review, we briefly describe the available scRNA-seq technologies together with their corresponding strengths and weaknesses. We discuss in depth how scRNA-seq can be used to deconvolve immune system heterogeneity by identifying novel distinct immune cell subsets in health and disease, characterizing stochastic heterogeneity within a cell population and building developmental 'trajectories' for immune cells. Finally, we discuss future directions of the field and present integrated approaches to complement molecular information from a single cell with studies of the environment, epigenetic state and cell lineage.

PMID:
28787399
DOI:
10.1038/nri.2017.76

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