Format

Send to

Choose Destination
ACS Synth Biol. 2017 Dec 15;6(12):2183-2190. doi: 10.1021/acssynbio.7b00234. Epub 2017 Aug 17.

Production of Functional Anti-Ebola Antibodies in Pichia pastoris.

Author information

1
Synthetic Biology Center, Department of Electrical Engineering and Computer Science, Department of Biological Engineering, 500 Technology Square, Massachusetts Institute of Technology , Cambridge, Massachusetts 02139, United States.
2
Department of Biology, Massachusetts Institute of Technology , Cambridge, Massachusetts 02139, United States.
3
Fraunhofer Institute for Molecular Biology and Applied Ecology IME , Forckenbeckstraβe 6, 52074 Aachen, Germany.
4
Department of Bioengineering, University of Illinois at Urbana-Champaign , Urbana, Illinois 61801, United States.
5
Institute for Molecular Biotechnology, RWTH Aachen University , Worringerweg 1, 52074 Aachen, Germany.

Abstract

The 2013-2016 Ebola outbreak highlighted the limited treatment options and lack of rapid response strategies for emerging pathogen outbreaks. Here, we propose an efficient development cycle using glycoengineered Pichia pastoris to produce monoclonal antibody cocktails against pathogens. To enable rapid genetic engineering of P. pastoris, we introduced a genomic landing pad for reliable recombinase-mediated DNA integration. We then created strains expressing each of the three monoclonal antibodies that comprise the ZMapp cocktail, and demonstrated that the secreted antibodies bind to the Ebola virus glycoprotein by immunofluorescence assay. We anticipate that this approach could accelerate the production of therapeutics against future pathogen outbreaks.

KEYWORDS:

Ebola; Pichia pastoris; antibodies; biologics

PMID:
28786662
DOI:
10.1021/acssynbio.7b00234
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for American Chemical Society
Loading ...
Support Center