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Eur J Oral Sci. 2017 Oct;125(5):361-370. doi: 10.1111/eos.12366. Epub 2017 Aug 8.

Orthodontically induced osteocyte apoptosis under different force magnitudes in rats: an immunohistochemical study.

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Department of Orthodontics, Faculty of Dentistry, Alexandria University, Alexandria, Egypt.
Department of Clinical Sciences, College of Medicine, University of Sharjah, Sharjah, United Arab Emirates.
Department of Pathology, Faculty of Medicine, Alexandria University, Alexandria, Egypt.
Department of Oral Biology, Faculty of Dentistry, Alexandria University, Alexandria, Egypt.


We investigated the effect of different force magnitudes on osteocyte apoptosis in a model of orthodontic tooth movement. Forty-nine male Sprague Dawley rats (7-9 wk of age) were divided into light- and heavy-force groups (n = 21 each group) and a control group (n = 7). A coil spring delivered pressure (either 10-15 g or 20-25 g) to the left maxillary first molar. The rats were sacrificed 1, 3, or 5 d after placement of the appliance. Sections of the maxillary first molars were immunostained for caspase-3. Upon force application, the number of apoptotic osteocytes significantly increased in the pressure side at 1 d and remained the same at 3 d and 5 d. However, there was no significant difference in the number of apoptotic osteocytes between the two force groups. We conclude that osteocyte apoptosis appears to increase under orthodontic loading, reaching a plateau after 1 d. However, osteocyte apoptosis seems to be independent of the magnitude of orthodontic forces tested.


caspase-3; orthodontic tooth movement; programmed cell death

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