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Eur J Oral Sci. 2017 Oct;125(5):361-370. doi: 10.1111/eos.12366. Epub 2017 Aug 8.

Orthodontically induced osteocyte apoptosis under different force magnitudes in rats: an immunohistochemical study.

Author information

1
Department of Orthodontics, Faculty of Dentistry, Alexandria University, Alexandria, Egypt.
2
Department of Clinical Sciences, College of Medicine, University of Sharjah, Sharjah, United Arab Emirates.
3
Department of Pathology, Faculty of Medicine, Alexandria University, Alexandria, Egypt.
4
Department of Oral Biology, Faculty of Dentistry, Alexandria University, Alexandria, Egypt.

Abstract

We investigated the effect of different force magnitudes on osteocyte apoptosis in a model of orthodontic tooth movement. Forty-nine male Sprague Dawley rats (7-9 wk of age) were divided into light- and heavy-force groups (n = 21 each group) and a control group (n = 7). A coil spring delivered pressure (either 10-15 g or 20-25 g) to the left maxillary first molar. The rats were sacrificed 1, 3, or 5 d after placement of the appliance. Sections of the maxillary first molars were immunostained for caspase-3. Upon force application, the number of apoptotic osteocytes significantly increased in the pressure side at 1 d and remained the same at 3 d and 5 d. However, there was no significant difference in the number of apoptotic osteocytes between the two force groups. We conclude that osteocyte apoptosis appears to increase under orthodontic loading, reaching a plateau after 1 d. However, osteocyte apoptosis seems to be independent of the magnitude of orthodontic forces tested.

KEYWORDS:

caspase-3; orthodontic tooth movement; programmed cell death

PMID:
28786155
DOI:
10.1111/eos.12366
[Indexed for MEDLINE]

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