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Acta Otolaryngol. 2017 Dec;137(12):1253-1259. doi: 10.1080/00016489.2017.1360515. Epub 2017 Aug 8.

Effects of pepsin A on heat shock protein 70 response in laryngopharyngeal reflux patients with chronic rhinosinusitis.

Author information

1
a Department of Oto-Rhino-Laryngology , West China Hospital, West China Medical School, Sichuan University , Chengdu , Sichuan , China.
2
b Department of Otolaryngology , Chengdu Renpin Otorhinolaryngological Hospital , Chengdu , Sichuan , China.
3
c Department of Biomedical Research , Nemours/Alfred I.duPont Hospital for Children , Wilmington , DE , USA.

Abstract

OBJECTIVES:

We investigated the relationship between laryngopharyngeal reflux (LPR) and chronic rhinosinusitis (CRS), and explored the effects of pepsin A on the level of heat shock protein 70 (HSP70) in CRS.

METHODS:

We included 23 CRS patients with nasal polyps (CRSwNP), 26 CRS patients without nasal polyps (CRSsNP) and nine normal controls to measure pepsin A levels in nasal secretions, blood plasma and nasal tissues, to measure HSP70 levels in nasal tissues, and to detect pepsinogen A, HSPA5, cyclo-oxygenase-2 (COX-2), and carbonic anhydrase III (CAIII) mRNA expression levels in nasal tissues.

RESULTS:

Pepsin A levels in nasal secretions were significantly higher in CRSwNP/CRSsNP patients than in controls. HSP70 levels were significantly increased in pepsin A-positive turbinate mucosa compared to controls (pā€‰<ā€‰.001). Similarly, HSP70 levels were significantly increased in pepsin A-positive polyp tissues than in pepsin A-negative polyp tissues (pā€‰=ā€‰.016). Furthermore, no association was found between the presence of pepsin A and HSPA5, COX-2, and CAIII mRNA expression levels.

CONCLUSIONS:

These results suggest that LPR may play a role in the development of CRS through pepsin A reflux, and increased HSP70 expression may be associated with the pathogenic mechanism of mucosal injury in CRS.

KEYWORDS:

Laryngopharyngeal reflux; chronic rhinosinusitis; heat shock protein 70; pepsin A

PMID:
28784015
DOI:
10.1080/00016489.2017.1360515
[Indexed for MEDLINE]

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