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FEBS Open Bio. 2017 Jun 29;7(8):1166-1177. doi: 10.1002/2211-5463.12252. eCollection 2017 Aug.

The proteomic profile of a mouse model of proliferative vitreoretinopathy.

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Department of Biochemistry and Molecular Biology Faculty of Medicine University of Debrecen Hungary.
Department of Ophthalmology Faculty of Medicine University of Szeged Hungary.
Department of Ophthalmology Oslo University Hospital and University of Oslo Norway.


Proliferative vitreoretinopathy (PVR) develops as a complication of retinal detachment surgery and represents a devastating condition leading to serious vision loss. A good animal model that permits extensive functional studies and drug testing is crucial in finding better therapeutic modalities for PVR. A previously established mouse model, using dispase injection, was analyzed from the proteomic point of view, examining global protein profile changes by 2D electrophoresis, image analysis and HPLC-tandem mass spectrometry-based protein identification. The easy applicability of the mouse model was used to study the role of transglutaminase 2 (TG2) in PVR formation by proteomic examination of dispase-induced TG2 knockout vitreous samples. Our data demonstrate that, despite the altered appearance of crystallin proteins, the lack of TG2 did not prevent the development of PVR.


2D electrophoresis; PVR; TG2; animal model; mass spectrometry

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