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Stem Cell Reports. 2017 Sep 12;9(3):985-998. doi: 10.1016/j.stemcr.2017.06.020. Epub 2017 Aug 3.

Activated Tissue-Resident Mesenchymal Stromal Cells Regulate Natural Killer Cell Immune and Tissue-Regenerative Function.

Author information

1
Department of Otorhinolaryngology, University Hospital Essen, University of Duisburg-Essen, Essen 45147, Germany.
2
Department of Orthopedics and Trauma Surgery, University Hospital Essen, University of Duisburg-Essen, Essen 45147, Germany.
3
Department of Dermatology, University Hospital Essen, University of Duisburg-Essen, Essen 45147, Germany.
4
Department of Otorhinolaryngology, University Hospital Essen, University of Duisburg-Essen, Essen 45147, Germany. Electronic address: sven.brandau@uk-essen.de.

Abstract

The interaction of mesenchymal stromal cells (MSCs) with natural killer (NK) cells is traditionally thought of as a static inhibitory model, whereby resting MSCs inhibit NK cell effector function. Here, we use a dynamic in vitro system of poly(I:C) stimulation to model the interaction of NK cells and tissue-resident MSCs in the context of infection or tissue injury. The experiments suggest a time-dependent system of regulation and feedback, where, at early time points, activated MSCs secrete type I interferon to enhance NK cell effector function, while at later time points TGF-β and IL-6 limit NK cell effector function and terminate inflammatory responses by induction of a regulatory senescent-like NK cell phenotype. Importantly, feedback of these regulatory NK cells to MSCs promotes survival, proliferation, and pro-angiogenic properties. Our data provide additional insight into the interaction of stromal cells and innate immune cells and suggest a model of time-dependent MSC polarization and licensing.

KEYWORDS:

Poly(I:C); TGF-β; TLR 3; mesenchymal stromal cell polarization; mucosal tissue; natural killer cell activation; senescence; type I interferon; viral infection

PMID:
28781075
PMCID:
PMC5599186
DOI:
10.1016/j.stemcr.2017.06.020
[Indexed for MEDLINE]
Free PMC Article

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