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Epidemiol Infect. 2017 Oct;145(13):2694-2700. doi: 10.1017/S0950268817001601. Epub 2017 Aug 7.

Molecular survey of Bartonella henselae and Bartonella clarridgeiae in pet cats across Japan by species-specific nested-PCR.

Author information

1
Laboratory of Veterinary Public Health, Department of Veterinary Medicine,College of Bioresource Sciences, Nihon University,1866 Kameino, Fujisawa, Kanagawa Prefecture, 252-0880,Japan.
2
Department of Veterinary Internal Medicine,Graduate School of Agricultural and Life Sciences, The University of Tokyo,1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657,Japan.
3
Laboratory of Molecular Immunology and Infectious Disease Joint Faculty of Veterinary Medicine, Yamaguchi University,1677-1 Yoshida, Yamaguchi Prefecture, 753-8515,Japan.
4
Laboratory of Small Animal Internal Medicine, Joint Faculty of Veterinary Medicine, Kagoshima University,1-21-24 Korimoto, Kagoshima, Kagoshima Prefecture, 890-0065,Japan.

Abstract

Cats are known to be the main reservoir for Bartonella henselae and Bartonella clarridgeiae, which are the agents of 'cat-scratch disease' in humans. In the present study, we investigated the prevalence of the two Bartonella species on 1754 cat bloods collected from all prefectures in Japan during 2007-2008 by a nested-polymerase chain reaction (PCR) targeting the 16S-23S rRNA internal transcribed spacer region. Overall, Bartonella DNA was detected in 4·6% (80/1754) of the cats examined. The nested-PCR showed that 48·8% (39/80) of the positive cats were infected with B. henselae mono-infection, 33·8% (27/80) with B. clarridgeiae mono-infection and 17·5% (14/80) were infected with both species. The prevalence (5·9%; 65/1103) of Bartonella infection in the western part of Japan was significantly higher than that (2·3%; 15/651) of eastern Japan (P < 0·001). Statistical analysis of the cats examined suggested a significant association between Bartonella infection and FeLV infection (OR = 1·9; 95% CI = 1·1-3·4), but not with FIV infection (OR = 1·6; 95% CI = 1·0-2·6).

KEYWORDS:

Bartonella clarridgeiae ; Bartonella henselae ; Japan; species-specific nested-PCR

PMID:
28780918
DOI:
10.1017/S0950268817001601
[Indexed for MEDLINE]

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