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Exp Parasitol. 2017 Oct;181:94-101. doi: 10.1016/j.exppara.2017.07.014. Epub 2017 Aug 2.

Effects of selected Indonesian plant extracts on E. cuniculi infection in vivo.

Author information

1
Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice, Czech Republic. Electronic address: casio@paru.cas.cz.
2
Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice, Czech Republic.
3
Department of Chemical Biology and Genetics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Olomouc, Czech Republic; Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacky University & Institute of Experimental Botany, Czech Academy of Sciences, Olomouc, Czech Republic.
4
Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice, Czech Republic; Faculty of Agriculture, University of South Bohemia in České Budějovice, České Budějovice, Czech Republic.
5
Faculty of Economics, University of South Bohemia in České Budějovice, České Budějovice, Czech Republic.
6
UMI - Saving of Pongidae Foundation, Brno, Czech Republic.
7
Department of Parasitology, Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta, Indonesia.
8
Department of Parasitology, Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta, Indonesia; Department of Botany and Zoology, Faculty of Science, Masaryk University, Brno, Czech Republic.

Abstract

The present study was conducted to evaluate the methanolic extracts from several plant leaves widely used in traditional medicine to cure digestive tract disorders and in the self-medication of wild animals such as non-human primates, namely Archidendron fagifolium, Diospyros sumatrana, Shorea sumatrana, and Piper betle leaves, with regard to their antimicrosporidial activity against Encephalitozoon cuniculi in immunocompetent BALB/c mice determined using molecular detection of microsporidial DNA (qPCR) in various tissues and body fluids of infected, treated mice. Of the plant extracts tested, Diospyros sumatrana provided the most promising results, reducing spore shedding by 88% compared to untreated controls. Moreover, total burden per 1 g of tissue in the D. sumatrana extract-treated group reached 87% reduction compared to untreated controls, which was comparable to the effect of the standard drug, Albendazole. This data represents the baseline necessary for further research focused on determining the structure, activity and modes of action of the active compounds, mainly of D. sumatrana, enabling subsequent development of antimicrosporidial remedies.

KEYWORDS:

Antifungal; Encephalitozoon cuniculi; In vivo; Molecular detection; Plant extract

PMID:
28779899
DOI:
10.1016/j.exppara.2017.07.014
[Indexed for MEDLINE]

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