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Mar Biotechnol (NY). 2017 Oct;19(5):497-516. doi: 10.1007/s10126-017-9769-1. Epub 2017 Aug 5.

Locus Mapping, Molecular Cloning, and Expression Analysis of rps6kb2, a Novel Metamorphosis-Related Gene in Chinese Tongue Sole (Cynoglossus semilaevis).

Liu Y1,2,3, Wei M1,2,3, Guo H2,3,4, Shao C2,3, Meng L2,3, Xu W2,3, Wang N2,3, Wang L2,3, Power DM4, Hou J5, Mahboob S6,7, Cui Z2,3,4, Yang Y2,3, Li Y2,3, Zhao F1,2,3, Chen S8,9,10.

Author information

1
Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214081, China.
2
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (CAFS), Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao, 266071, China.
3
Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China.
4
College of Fisheries and Life Science, Shanghai Ocean University, Ministry of Education, Shanghai, 201306, China.
5
Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao, 066100, China.
6
Department of Zoology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia.
7
Department of Zoology, GC University, Faisalabad, 38000, Pakistan.
8
Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214081, China. chensl@ysfri.ac.cn.
9
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (CAFS), Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao, 266071, China. chensl@ysfri.ac.cn.
10
Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China. chensl@ysfri.ac.cn.

Abstract

Flatfish metamorphosis denotes the extraordinary transformation of a symmetric pelagic larva into an asymmetric benthic juvenile. This unique process involves eye migration, a 90° rotation in posture, and asymmetrical pigmentation for adaptation to a benthic lifestyle. In the present study, we used genetics to map a metamorphosis-related locus (q-10M) in the male linkage group (LG10M), a small interval of 0.9 cM corresponding to a 1.8 M-bp physical area in chromosome 9 in the Chinese tongue sole (Cynoglossus semilaevis). Combined with single-marker analysis, ribosomal protein S6 kinase 2 (rps6kb2) a member of the family of AGC kinases was identified as a novel metamorphosis-related candidate gene. Its expression pattern during metamorphosis was determined by quantitative RT-PCR and whole-mount in situ hybridization analysis. rps6kb2 gene was significantly expressed in metamorphic climax stage larvae and distributed in all the tissues transforming during metamorphosis, including tail, jaw, eye and skin of larvae. The results suggest that rps6kb2 has a general role in tissue transformations during flatfish metamorphosis including tail changes, skull remodeling, eye migration, and asymmetrical pigmentation.

KEYWORDS:

Cynoglossus semilaevis; Locus mapping; Metamorphosis; Ribosomal protein S6 kinase 2 (rps6kb2); Simple sequence repeat (SSR)

PMID:
28779262
DOI:
10.1007/s10126-017-9769-1
[Indexed for MEDLINE]

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