Format

Send to

Choose Destination
Sci Rep. 2017 Aug 4;7(1):7340. doi: 10.1038/s41598-017-07494-0.

Bee-derived antibacterial peptide, defensin-1, promotes wound re-epithelialisation in vitro and in vivo.

Author information

1
Laboratory of Apidology and Apitherapy, Institute of Molecular Biology, Slovak Academy of Sciences, Dubravska cesta 21, 845 51, Bratislava, Slovakia.
2
Institute of Zoology, Slovak Academy of Sciences, Dubravska cesta 9, 845 06, Bratislava, Slovakia.
3
Department of Microbiology, Faculty of Medicine, Slovak Medical University, Limbova 12, 833 03, Bratislava, Slovakia.
4
Faculty of Natural Sciences, Comenius University, Ilkovicova 6, 842 15, Bratislava, Slovakia.
5
DiSIT-Dipartimento di Scienze e Innovazione Tecnologica, University of Piemonte Orientale, Viale Teresa Michel 11, Alessandria, 15121, Italy.
6
DiSIT-Dipartimento di Scienze e Innovazione Tecnologica, University of Piemonte Orientale, piazza Sant'Eusebio 5, Vercelli, 13100, Italy.
7
Cytopahtos Laboratory, Kutuzovova 23, 831 03, Bratislava, Slovakia.
8
Institute of Chemistry, Slovak Academy of Sciences, Dubravska cesta 9, 845 38, Bratislava, Slovakia.
9
Laboratory of Apidology and Apitherapy, Institute of Molecular Biology, Slovak Academy of Sciences, Dubravska cesta 21, 845 51, Bratislava, Slovakia. juraj.majtan@savba.sk.
10
Institute of Zoology, Slovak Academy of Sciences, Dubravska cesta 9, 845 06, Bratislava, Slovakia. juraj.majtan@savba.sk.
11
Department of Microbiology, Faculty of Medicine, Slovak Medical University, Limbova 12, 833 03, Bratislava, Slovakia. juraj.majtan@savba.sk.

Abstract

Royal jelly (RJ) has successfully been used as a remedy in wound healing. RJ has multiple effects, including antibacterial, anti-inflammatory and immunomodulatory activities, in various cell types. However, no component(s) (other than antibacterial) have been identified in RJ-accelerated wound healing. In this study, we demonstrate that keratinocytes are responsible for the elevated production of matrix metalloproteinase-9 (MMP-9) after incubation with a water extract of RJ. Furthermore, the keratinocyte migration and wound closure rates were significantly increased in the presence of RJ extract. MMP-9 production was reduced significantly following proteinase K treatment but remained stable after heat treatment, indicating that active component(s) have a proteinous character. To identify the component responsible for inducing MMP-9 production, RJ extract was fractionated using C18 RP-HPLC. In fractions exhibiting stimulatory activity, we immunochemically detected the bee-derived antibacterial peptide, defensin-1. Defensin-1 was cloned, and recombinant peptide was produced in a baculoviral expression system. Defensin-1 stimulated MMP-9 secretion from keratinocytes and increased keratinocyte migration and wound closure in vitro. In addition, defensin-1 promoted re-epithelisation and wound closure in uninfected excision wounds. These data indisputably demonstrate that defensin-1, a regular but concentration variable factor found in honey and RJ, contributes to cutaneous wound closure by enhancing keratinocyte migration and MMP-9 secretion.

PMID:
28779102
PMCID:
PMC5544694
DOI:
10.1038/s41598-017-07494-0
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center