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Fungal Genet Biol. 2017 Oct;107:24-30. doi: 10.1016/j.fgb.2017.07.007. Epub 2017 Aug 1.

FvSet2 regulates fungal growth, pathogenicity, and secondary metabolism in Fusarium verticillioides.

Author information

1
Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Education, Nanjing, China.
2
China Rural Technology Development Center, China.
3
Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Education, Nanjing, China. Electronic address: hjwu@njau.edu.cn.

Abstract

Histone H3 lysine 36 methylation (H3K36me) is generally associated with activation of gene expression in most eukaryotic cells. However, the function of H3K36me in filamentous fungi is largely unknown. Set2 is the sole lysine histone methyltransferase (KHMTase) enzyme responsible for the methylation of H3K36 in Saccharomyces cerevisiae. In the current study, we identified a single ortholog of S. cerevisiae Set2 in Fusarium verticillioides. We report that FvSet2 is responsible for the trimethylation of H3K36 (H3K36me3). The FvSET2 deletion mutant (ΔFvSet2) showed significant defects in vegetative growth, FB1 biosynthesis, pigmentation, and fungal virulence. Furthermore, trimethylation of H3K36 was found to be important for active transcription of genes involved in FB1 and bikaverin biosyntheses. These data indicate that FvSet2 plays an important role in the regulation of secondary metabolism, vegetative growth and fungal virulence in F. verticillioides.

KEYWORDS:

FB(1); Fungal virulence; Fusarium verticillioides; FvSet2; H3K36 trimethylation

PMID:
28778753
DOI:
10.1016/j.fgb.2017.07.007
[Indexed for MEDLINE]

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