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Fertil Steril. 2017 Aug;108(2):269-276.e1. doi: 10.1016/j.fertnstert.2017.06.004.

Effects of high progesterone level on the day of human chorionic gonadotrophin administration in in vitro fertilization cycles on epigenetic modification of endometrium in the peri-implantation period.

Author information

1
Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Hong Kong; Reproduction and Development, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Hong Kong.
2
Department of Obstetrics and Gynaecology, Center for Reproductive Medicine, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou, People's Republic of China.
3
Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Hong Kong.
4
Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Hong Kong; Reproduction and Development, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Hong Kong; School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong.
5
Department of Obstetrics and Gynaecology, Center for Reproductive Medicine, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou, People's Republic of China. Electronic address: zhangsongying@126.com.

Abstract

OBJECTIVE:

To investigate the effects of high P level on the day of hCG administration in IVF cycles on epigenetic modifications of endometrium in the peri-implantation period.

DESIGN:

Retrospective study.

SETTING:

University assisted reproductive unit.

PATIENT(S):

Forty patients were recruited: 20 women with high P levels were compared with 20 women with normal P levels.

INTERVENTION(S):

Endometrial tissues were collected 7 days after hCG administration in women with normal or high P levels on the day of hCG administration. Immunohistochemical staining of DNA methylation (5-methylcytosine, 5-mC), histone methylation (H3K4me2/3, H3K9me2, H3K27me3), and histone acetylation (H3K4ac, H3K9ac) was performed.

MAIN OUTCOME MEASURE(S):

A semiquantitative analysis was performed in the luminal epithelium, glandular epithelium, and stroma, separately. Correlations between P, estrogen levels, and epigenetic markers were compared.

RESULT(S):

In luminal epithelium, the expression of H3K9me2 in the high P group was significantly higher than that in the normal P group. In glandular epithelium, the expression of 5-mC, H3K9me2, and H3K9ac in the high P group was significantly higher than that in the normal P group. In stroma, the expression of H3K27me3 in the high P group was significantly higher than that in the normal P group. Expression of 5-mC in glandular epithelium, H3k9me2 in glandular and luminal epithelium, and H3K27me3 in stroma was significantly correlated with P levels. H3k9me2 in glandular epithelium was significantly correlated with estrogen levels.

CONCLUSION(S):

High P level on the day of hCG administration in IVF cycles affects epigenetic modifications in three compartments of endometrium in the peri-implantation period. The epigenetic markers' expression was mainly correlated with P level. The altered epigenetic modification status in the endometrium may disrupt the endometrial receptivity in women with high P on the day of hCG administration.

KEYWORDS:

Endometrium; epigenetics; progesterone

[Indexed for MEDLINE]

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