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Sci Rep. 2017 Aug 3;7(1):7260. doi: 10.1038/s41598-017-07313-6.

A conserved neuronal DAF-16/FoxO plays an important role in conveying pheromone signals to elicit repulsion behavior in Caenorhabditis elegans.

Park D1,2, Hahm JH1,2,3, Park S4, Ha G5, Chang GE5, Jeong H1,2, Kim H2, Kim S1,2,3, Cheong E5, Paik YK6,7,8.

Author information

1
Department of Biochemistry, Yonsei University, Seoul, Korea.
2
Yonsei Proteome Research Center, Yonsei University, Seoul, Korea.
3
Center for Plant Aging Research, Institute for Basic Science (IBS), Daegu, 42988, Republic of Korea.
4
Department of Integrated Omics for Biomedical Science, Yonsei University, Seoul, Korea.
5
Department of Biotechnology, and College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.
6
Department of Biochemistry, Yonsei University, Seoul, Korea. paikyk@yonsei.ac.kr.
7
Yonsei Proteome Research Center, Yonsei University, Seoul, Korea. paikyk@yonsei.ac.kr.
8
Department of Integrated Omics for Biomedical Science, Yonsei University, Seoul, Korea. paikyk@yonsei.ac.kr.

Abstract

Animals use pheromones as a conspecific chemical language to respond appropriately to environmental changes. The soil nematode Caenorhabditis elegans secretes ascaroside pheromones throughout the lifecycle, which influences entry into dauer phase in early larvae, in addition to sexual attraction and aggregation. In adult hermaphrodites, pheromone sensory signals perceived by worms usually elicit repulsion as an initial behavioral signature. However, the molecular mechanisms underlying neuronal pheromone sensory process from perception to repulsion in adult hermaphrodites remain poorly understood. Here, we show that pheromone signals perceived by GPA-3 is conveyed through glutamatergic neurotransmission in which neuronal DAF-16/FoxO plays an important modulatory role by controlling glutaminase gene expression. We further provide evidence that this modulatory role for DAF-16/FoxO seems to be conserved evolutionarily by electro-physiological study in mouse primary hippocampal neurons that are responsible for glutamatergic neurotransmission. These findings provide the basis for understanding the nematode pheromone signaling, which seems crucial for adaptation of adult hermaphrodites to changes in environmental condition for survival.

PMID:
28775361
PMCID:
PMC5543152
DOI:
10.1038/s41598-017-07313-6
[Indexed for MEDLINE]
Free PMC Article

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