Send to

Choose Destination
Neuron. 2017 Aug 2;95(3):504-529. doi: 10.1016/j.neuron.2017.06.050.

Silencing Neurons: Tools, Applications, and Experimental Constraints.

Author information

Research Group Synaptic Wiring and Information Processing, Center for Molecular Neurobiology Hamburg, Falkenried 94, 20251 Hamburg, Germany.
Department of Neurobiology, Weizmann Institute of Science, Rehovot 76100, Israel.
Department of Neurobiology, Weizmann Institute of Science, Rehovot 76100, Israel. Electronic address:


Reversible silencing of neuronal activity is a powerful approach for isolating the roles of specific neuronal populations in circuit dynamics and behavior. In contrast with neuronal excitation, for which the majority of studies have used a limited number of optogenetic and chemogenetic tools, the number of genetically encoded tools used for inhibition of neuronal activity has vastly expanded. Silencing strategies vary widely in their mechanism of action and in their spatial and temporal scales. Although such manipulations are commonly applied, the design and interpretation of neuronal silencing experiments present unique challenges, both technically and conceptually. Here, we review the most commonly used tools for silencing neuronal activity and provide an in-depth analysis of their mechanism of action and utility for particular experimental applications. We further discuss the considerations that need to be given to experimental design, analysis, and interpretation of collected data. Finally, we discuss future directions for the development of new silencing approaches in neuroscience.


archaerhodopsin; channelrhodopsin; chemogenetics; halorhodopsin; light-activated G-protein-coupled receptors; light-gated anion channelrhodopsins; neuronal silencing; optogenetics; synaptic transmission

[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center