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Int J Mol Sci. 2017 Aug 3;18(8). pii: E1698. doi: 10.3390/ijms18081698.

Scar Prevention and Enhanced Wound Healing Induced by Polydeoxyribonucleotide in a Rat Incisional Wound-Healing Model.

Author information

1
Department of Plastic and Reconstructive Surgery, School of Medicine & Institute for Medical Science, Keimyung University, Dongsan Medical Center, Daegu 41931, Korea. psjeong0918@gmail.com.
2
Department of Plastic and Reconstructive Surgery, Institute for Human Tissue Restoration, Yonsei University Health System, Severance Hospital, Seoul 03722, Korea. cheniya@yuhs.ac.
3
Department of Plastic and Reconstructive Surgery, Institute for Human Tissue Restoration, Gangnam Yonsei University Health System, Severance Hospital, Seoul 06273, Korea. tsroh@yuhs.ac.
4
Department of Plastic and Reconstructive Surgery, School of Medicine & Institute for Medical Science, Keimyung University, Dongsan Medical Center, Daegu 41931, Korea. med69@dsmc.or.kr.
5
Department of Dermatology and Cutaneous Biology Research Institute, Severance Hospital, Yonsei University College of Medicine, Seoul 03722, Korea. juhee@yuhs.ac.
6
Department of Plastic and Reconstructive Surgery, Institute for Human Tissue Restoration, Yonsei University Health System, Severance Hospital, Seoul 03722, Korea. pswjlee@yuhs.ac.

Abstract

High-mobility group box protein-1 (HMGB-1) plays a central role in the inflammatory network, and uncontrolled chronic inflammation can lead to excessive scarring. The aim of this study was to evaluate the anti-inflammatory effects of polydeoxyribonucleotide (PDRN) on scar formation. Sprague-Dawley rats (n = 30) underwent dorsal excision of the skin, followed by skin repair. PDRN (8 mg/kg) was administered via intraperitoneal injection for three (PDRN-3 group, n = 8) or seven (PDRN-7 group, n = 8) days, and HMGB-1 was administered via intradermal injection in addition to PDRN treatment for three days (PDRN-3+HMGB-1 group; n = 6). The scar-reducing effects of PDRN were evaluated in the internal scar area and by inflammatory cell counts using histology and immunohistochemistry. Western blot, immunohistochemistry and immunofluorescence assays were performed to observe changes in type I and type III collagen and the expression of HMGB-1 and CD45. Treatment with PDRN significantly reduced the scar area, inflammatory cell infiltration and the number of CD45-positive cells. In addition, the increased expression of HMGB-1 observed in the sham group was significantly reduced after treatment with PDRN. Rats administered HMGB-1 in addition to PDRN exhibited scar areas with inflammatory cell infiltration similar to the sham group, and the collagen synthesis effects of PDRN were reversed. In summary, PDRN exerts anti-inflammatory and collagen synthesis effects via HMGB-1 suppression, preventing scar formation. Thus, we believe that the anti-inflammatory and collagen synthesis effects of PDRN resulted in faster wound healing and decreased scar formation.

KEYWORDS:

cicatrix, inflammation; polydeoxyribonucleotide; rats; wounds and injuries

PMID:
28771195
PMCID:
PMC5578088
DOI:
10.3390/ijms18081698
[Indexed for MEDLINE]
Free PMC Article

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