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Lab Chip. 2017 Sep 12;17(18):3037-3042. doi: 10.1039/c7lc00552k.

Cell-free extract based optimization of biomolecular circuits with droplet microfluidics.

Author information

1
Department of Computing and Mathematical Sciences, California Institute of Technology, Pasadena, USA.

Abstract

Engineering an efficient biomolecular circuit often requires time-consuming iterations of optimization. Cell-free protein expression systems allow rapid testing of biocircuits in vitro, speeding the design-build-test cycle of synthetic biology. In this paper, we combine this with droplet microfluidics to densely scan a transcription-translation biocircuit space. Our system assays millions of parameter combinations per hour, providing a detailed map of function. The ability to comprehensively map biocircuit parameter spaces allows accurate modeling to predict circuit function and identify optimal circuits and conditions.

PMID:
28770936
DOI:
10.1039/c7lc00552k
[Indexed for MEDLINE]

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