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Clin Exp Rheumatol. 2018 Jan-Feb;36(1):121-126. Epub 2017 Jul 27.

Prevalence of anti-DFS70 antibodies in patients with and without systemic autoimmune rheumatic diseases.

Author information

1
Zabludowitz Centre for Autoimmune Diseases; Department of Internal Medicine 'B'; Rheumatology Unit, Sheba Medical Centre, Tel Hashomer, Israel.
2
Zabludowitz Centre for Autoimmune Diseases, Sheba Medical Centre, Tel Hashomer, Israel.
3
Zabludowitz Centre for Autoimmune Diseases; Department of Internal Medicine 'B', Sheba Medical Centre, Tel Hashomer; and Sackler Faculty of Medicine, Tel Aviv University, Israel.
4
Rheumatology Unit, Sheba Medical Centre, Tel Hashomer; and Sackler Faculty of Medicine, Tel Aviv University, Israel.
5
Department of Internal Medicine 'B', Sheba Medical Centre, Tel Hashomer, Israel.
6
Zabludowitz Centre for Autoimmune Diseases, Sheba Medical Centre, Tel Hashomer; and Sackler Faculty of Medicine, Tel Aviv University, Israel.
7
Research and Development, Inova Diagnostics, San Diego, CA, USA.
8
Zabludowitz Centre for Autoimmune Diseases, Sheba Medical Centre, Tel Hashomer; Incumbent of the Laura Schwarz-Kipp Chair for Research of Autoimmune Diseases, Sackler Faculty of Medicine, Tel Aviv University, Israel. shoenfel@post.tau.ac.il.

Abstract

OBJECTIVES:

Autoantibodies to the dense fine speckled 70 (DFS70) antigen are common among antinuclear antibodies (ANA) positive healthy individuals (HI). We assessed the prevalence of anti-DFS70 antibodies in patients with and without ANA-associated rheumatic diseases (AARDs) by two methods: chemiluminescent immunoassay (CIA) and an indirect immunofluorescence (IIF) assay based on immunoadsorption for DFS70.

METHODS:

Fifty-one ANA-positive sera samples from patients with confirmed clinical diagnosis of AARD, 92 samples from HI and 85 samples submitted to a reference laboratory for routine ANA testing were evaluated for the presence of anti-DFS70 antibodies. The samples were evaluated by QUANTA Flash DFS70 CIA using BIO-FLASH instrument and by NOVA Lite selected HEp-2 kit on NOVA View - an automated IIF system. Sera with DFS positive pattern were pre-absorbed with highly purified human DFS70 antigen, and then tested again.

RESULTS:

Twenty-four samples (10.5%) tested by QUANTA Flash DFS70 CIA were positive for anti-DFS70 antibodies. The prevalence of monospecific anti-DFS70 antibodies was significantly higher in healthy subjects than in patients with AARDs (10.9% vs. 1.9%, p=0.02). The frequency of anti-DFS70 antibodies in samples submitted for routine ANA testing was 15.2%. A very good agreement was found between CIA and the DFS pattern identified by the automated HEp-2 IIF (kappa=0.97). In 80% of the samples obtained from patients without AARDs, immunoadsorption effectively inhibited the anti-DFS70 antibodies.

CONCLUSIONS:

The data confirm that mono-specific anti-DFS70 antibodies are a strong discriminator between ANA positive HI and AARD patients, and their evaluation should be included in ANA testing algorithms.

PMID:
28770702
[Indexed for MEDLINE]
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