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Regen Med. 2017 Jul;12(5):525-539. doi: 10.2217/rme-2017-0023. Epub 2017 Aug 3.

Host cell recruitment patterns by bone morphogenetic protein-2 releasing hyaluronic acid hydrogels in a mouse subcutaneous environment.

Author information

1
Department of Experimental Medicine (DIMES), University of Genoa & IRCCS Policlinico San Martino, Genoa, Italy.
2
Endodontics, Conservative Dentistry Department, Faculty of Dentistry, Alexandria University, Alexandria, Egypt.
3
Department of Chemistry, Angstrom Laboratory, Uppsala University, Uppsala, Sweden.

Abstract

AIM:

This study aimed to identify host cell recruitment patterns in a mouse model in response to rhBMP-2 releasing hyaluronic acid hydrogels and influence of added nano-hydroxyapatite particles on rhBMP-2 release and pattern of bone formation.

MATERIALS & METHODS:

Implanted gels were retrieved after implantation and cells were enzymatically dissociated for flow cytometric analysis. Percentages of macrophages, progenitor endothelial cells and putative mesenchymal stem cells were measured. Implants were evaluated for BMP-2 release by ELISA and by histology to monitor tissue formation.

RESULTS & CONCLUSION:

Hyaluronic acid+BMP-2 gels influenced the inflammatory response in the bone healing microenvironment. Host-derived putative mesenchymal stem cells were major contributors. Addition of hydroxyapatite nanoparticles modified the release pattern of rhBMP-2, resulting in enhanced bone formation.

KEYWORDS:

BMP-2; cell recruitment; controlled release; endothelial progenitor cells; hyaluronic acid hydrogel; macrophages; putative mesenchymal stem cells

PMID:
28770657
DOI:
10.2217/rme-2017-0023
[Indexed for MEDLINE]

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