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J Virol Methods. 1986 Sep;14(2):133-40.

Isolation of dengue viruses by intracerebral inoculation of mosquito larvae.

Abstract

The isolation of dengue viruses from clinical specimens has always posed a particularly difficult problem. The use of invertebrate cell cultures such as AP-61 and C6/36 has reduced the time required for definitive diagnosis to within a week. More recently, inoculation of adult mosquitoes has been used but it requires more than a week to reach a confirmed laboratory diagnosis. We describe a method using intracerebral inoculation of immobilized fourth instar of Toxorhynchites splendens larvae for the isolation of dengue viruses from clinical specimens which yields results within a few days following incubation at 32 degrees C.

PMID:
2877004
DOI:
10.1016/0166-0934(86)90044-3
[Indexed for MEDLINE]

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