Send to

Choose Destination
Sci Rep. 2017 Aug 2;7(1):7110. doi: 10.1038/s41598-017-07672-0.

Human adipose-derived mesenchymal stem cells seeded into a collagen-hydroxyapatite scaffold promote bone augmentation after implantation in the mouse.

Author information

IOM Ricerca, Viagrande, (CT), Italy.
Department of Biomedical and Biotechnological Sciences, University of Catania, Catania, Italy.
Fin-Ceramica Faenza, Faenza, (RA), Italy.
Department of Medical and Surgical Sciences and Advanced Technologies, G.F. Ingrassia, "Policlinico Vittorio Emanuele", Anatomic Pathology Section, University of Catania, Catania, Italy.
Department of Experimental Oncology, Mediterranean Institute of Oncology, Viagrande, Italy.
IOM Ricerca, Viagrande, (CT), Italy.
Department of Biomedical and Biotechnological Sciences, University of Catania, Catania, Italy.


Traumatic injury or surgical excision of diseased bone tissue usually require the reconstruction of large bone defects unable to heal spontaneously, especially in older individuals. This is a big challenge requiring the development of biomaterials mimicking the bone structure and capable of inducing the right commitment of cells seeded within the scaffold. In particular, given their properties and large availability, the human adipose-derived stem cells are considered as the better candidate for autologous cell transplantation. In order to evaluate the regenerative potential of these cells along with an osteoinductive biomaterial, we have used collagen/hydroxyapatite scaffolds to test ectopic bone formation after subcutaneous implantation in mice. The process was analysed both in vivo, by Fluorescent Molecular Tomography (FMT), and ex vivo, to evaluate the formation of bone and vascular structures. The results have shown that the biomaterial could itself be able of promoting differentiation of host cells and bone formation, probably by means of its intrinsic chemical and structural properties, namely the microenvironment. However, when charged with human mesenchymal stem cells, the ectopic bone formation within the scaffold was increased. We believe that these results represent an important advancement in the field of bone physiology, as well as in regenerative medicine.

[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center