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J Clin Periodontol. 2017 Dec;44(12):1274-1284. doi: 10.1111/jcpe.12788. Epub 2017 Nov 21.

Exploring the microbiome of healthy and diseased peri-implant sites using Illumina sequencing.

Author information

1
Section of Periodontology, Faculty of Odontology, University Complutense of Madrid, Madrid, Spain.
2
Department of Dental Ecology, University of North Carolina at Chapel Hill School of Dentistry, Chapel Hill, NC, USA.
3
Department of Periodontology, University of North Carolina at Chapel Hill School of Dentistry, Chapel Hill, NC, USA.
4
Department of Applied Oral Sciences, The Forsyth Institute, Cambridge, MA, USA.
5
Department of Dental Medicine, Karolinska Institute, Stockholm, Sweden.
6
Clinic of Fixed and Removable Prosthodontics and Dental Material Science, Center of Dental Medicine, University of Zürich, Zürich, Switzerland.

Abstract

AIM:

To compare the microbiome of healthy (H) and diseased (P) peri-implant sites and determine the core peri-implant microbiome.

MATERIALS AND METHODS:

Submucosal biofilms from 32 H and 35 P sites were analysed using 16S rRNA sequencing (MiSeq, Illumina), QIIME and HOMINGS. Differences between groups were determined using principal coordinate analysis (PCoA), t tests and Wilcoxon rank sum test and FDR-adjusted. The peri-implant core microbiome was determined.

RESULTS:

PCoA showed partitioning between H and P at all taxonomic levels. Bacteroidetes, Spirochetes and Synergistetes were higher in P, while Actinobacteria prevailed in H (p < .05). Porphyromonas and Treponema were more abundant in P while Rothia and Neisseria were higher in H (p < .05). The core peri-implant microbiome contained Fusobacterium, Parvimonas and Campylobacter sp. T. denticola, and P. gingivalis levels were higher in P, as well as F. alocis, F. fastidiosum and T. maltophilum (p < .05).

CONCLUSION:

The peri-implantitis microbiome is commensal-depleted and pathogen-enriched, harbouring traditional and new pathogens. The core peri-implant microbiome harbours taxa from genera often associated with periodontal inflammation.

KEYWORDS:

DNA ; dental implant; microbiome; peri-implantitis; periodontal; sequencing

PMID:
28766745
DOI:
10.1111/jcpe.12788
[Indexed for MEDLINE]
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