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J Cell Biol. 2017 Oct 2;216(10):3387-3403. doi: 10.1083/jcb.201704045. Epub 2017 Aug 1.

FiloQuant reveals increased filopodia density during breast cancer progression.

Author information

1
Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, Turku, Finland guillaume.jacquemet@utu.fi.
2
Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, Turku, Finland.
3
Center for Human Immunobiology, Texas Children's Hospital, Houston, TX.
4
Department of Pediatrics, Pathology and Immunology, Baylor College of Medicine, Houston, TX.
5
Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, Turku, Finland johanna.ivaska@utu.fi.
6
Department of Biochemistry, University of Turku, Turku, Finland.

Abstract

Defective filopodia formation is linked to pathologies such as cancer, wherein actively protruding filopodia, at the invasive front, accompany cancer cell dissemination. Despite wide biological significance, delineating filopodia function in complex systems remains challenging and is particularly hindered by lack of compatible methods to quantify filopodia properties. Here, we present FiloQuant, a freely available ImageJ plugin, to detect filopodia-like protrusions in both fixed- and live-cell microscopy data. We demonstrate that FiloQuant can extract quantifiable information, including protrusion dynamics, density, and length, from multiple cell types and in a range of microenvironments. In cellular models of breast ductal carcinoma in situ, we reveal a link between filopodia formation at the cell-matrix interface, in collectively invading cells and 3D tumor spheroids, and the in vitro invasive capacity of the carcinoma. Finally, using intravital microscopy, we observe that tumor spheroids display filopodia in vivo, supporting a potential role for these protrusions during tumorigenesis.

PMID:
28765364
PMCID:
PMC5626550
DOI:
10.1083/jcb.201704045
[Indexed for MEDLINE]
Free PMC Article

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