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Cold Spring Harb Protoc. 2017 Aug 1;2017(8):pdb.top077735. doi: 10.1101/pdb.top077735.

Budding Yeast Strains and Genotype-Phenotype Mapping.

Author information

1
IRCAN, CNRS UMR 6267, INSERM U998, University of Nice, 06107 Nice, France.
2
Department of Chemistry and Molecular Biology, University of Gothenburg, 40530 Gothenburg, Sweden.
3
Centre for Integrative Genetics (CIGENE), Department of Animal and Aquacultural Sciences, Norwegian University of Life Sciences (UMB), 1432 Ås, Norway.
4
Department of Chemistry and Molecular Biology, University of Gothenburg, 40530 Gothenburg, Sweden; anders.blomberg@cmb.gu.se.

Abstract

A small number of well-studied laboratory strains of Saccharomyces cerevisiae, mostly derived from S288C, are used in yeast research. Although powerful, studies for understanding S288C do not always capture the phenotypic essence or the genetic complexity of S. cerevisiae biology. This is particularly problematic for multilocus phenotypes identified in laboratory strains because these loci have never been jointly exposed to natural selection and the corresponding phenotypes do not represent optimization for any particular purpose or environment. Isolation and sequencing of new natural yeast strains also reveal that the total sequence diversity of the S. cerevisiae global population is poorly sampled in common laboratory strains. Here we discuss methodologies required for using the natural genetic variation in yeast to complete a genotype-phenotype map.

PMID:
28765302
DOI:
10.1101/pdb.top077735
[Indexed for MEDLINE]

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