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Development. 2017 Aug 1;144(15):2748-2763. doi: 10.1242/dev.146811.

Epigenetic resetting of human pluripotency.

Author information

1
Wellcome Trust - Medical Research Council Cambridge Stem Cell Institute, University of Cambridge, Cambridge CB2 1QR, UK gg251@cam.ac.uk austin.smith@cscr.cam.ac.uk.
2
Epigenetics Programme, Babraham Institute, Cambridge CB22 3AT, UK.
3
Wellcome Trust - Medical Research Council Cambridge Stem Cell Institute, University of Cambridge, Cambridge CB2 1QR, UK.
4
Centre for Stem Cell Biology, Department of Biomedical Science, University of Sheffield, Sheffield S10 2TN, UK.
5
Department of Biological Chemistry, University of California, Los Angeles, CA 90095, USA.
6
Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA.
7
Wellcome Trust Sanger Institute, Hinxton CB10 1SA, UK.
8
Department of Biochemistry, University of Cambridge, Cambridge CB2 1GA, UK.

Abstract

Much attention has focussed on the conversion of human pluripotent stem cells (PSCs) to a more naïve developmental status. Here we provide a method for resetting via transient histone deacetylase inhibition. The protocol is effective across multiple PSC lines and can proceed without karyotype change. Reset cells can be expanded without feeders with a doubling time of around 24 h. WNT inhibition stabilises the resetting process. The transcriptome of reset cells diverges markedly from that of primed PSCs and shares features with human inner cell mass (ICM). Reset cells activate expression of primate-specific transposable elements. DNA methylation is globally reduced to a level equivalent to that in the ICM and is non-random, with gain of methylation at specific loci. Methylation imprints are mostly lost, however. Reset cells can be re-primed to undergo tri-lineage differentiation and germline specification. In female reset cells, appearance of biallelic X-linked gene transcription indicates reactivation of the silenced X chromosome. On reconversion to primed status, XIST-induced silencing restores monoallelic gene expression. The facile and robust conversion routine with accompanying data resources will enable widespread utilisation, interrogation, and refinement of candidate naïve cells.

KEYWORDS:

Differentiation; Human embryo; Methylome; Pluripotent stem cells; Reprogramming

PMID:
28765214
PMCID:
PMC5560041
DOI:
10.1242/dev.146811
[Indexed for MEDLINE]
Free PMC Article

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