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Transl Psychiatry. 2017 Aug 1;7(8):e1187. doi: 10.1038/tp.2017.171.

BECon: a tool for interpreting DNA methylation findings from blood in the context of brain.

Author information

1
Department of Medical Genetics, University of British Columbia, Vancouver, BC, Canada.
2
Centre for Molecular Medicine and Therapeutics, BC Children's Hospital, Vancouver, BC, Canada.
3
Ludmer Centre for Neuroinformatics and Mental Health, Douglas Mental Health University Institute, McGill University, Montreal, QC, Canada.
4
Singapore Institute for Clinical Sciences, Singapore.
5
Canadian Institute for Advanced Research, Toronto, ON, Canada.
6
Department of Psychiatry, McGill University, Montreal, QC, Canada.

Abstract

Tissue differences are one of the largest contributors to variability in the human DNA methylome. Despite the tissue-specific nature of DNA methylation, the inaccessibility of human brain samples necessitates the frequent use of surrogate tissues such as blood, in studies of associations between DNA methylation and brain function and health. Results from studies of surrogate tissues in humans are difficult to interpret in this context, as the connection between blood-brain DNA methylation is tenuous and not well-documented. Here, we aimed to provide a resource to the community to aid interpretation of blood-based DNA methylation results in the context of brain tissue. We used paired samples from 16 individuals from three brain regions and whole blood, run on the Illumina 450 K Human Methylation Array to quantify the concordance of DNA methylation between tissues. From these data, we have made available metrics on: the variability of cytosine-phosphate-guanine dinucleotides (CpGs) in our blood and brain samples, the concordance of CpGs between blood and brain, and estimations of how strongly a CpG is affected by cell composition in both blood and brain through the web application BECon (Blood-Brain Epigenetic Concordance; https://redgar598.shinyapps.io/BECon/). We anticipate that BECon will enable biological interpretation of blood-based human DNA methylation results, in the context of brain.

PMID:
28763057
PMCID:
PMC5611738
DOI:
10.1038/tp.2017.171
[Indexed for MEDLINE]
Free PMC Article

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