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Proc Natl Acad Sci U S A. 2017 Aug 15;114(33):8728-8733. doi: 10.1073/pnas.1704020114. Epub 2017 Jul 31.

Printed droplet microfluidics for on demand dispensing of picoliter droplets and cells.

Author information

1
Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94143.
2
Department of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences, University of California, San Francisco, CA 94158.
3
UC Berkeley-UCSF Graduate Program in Bioengineering, University of California, San Francisco, CA 94158.
4
Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94143; arabate@gmail.com zev.gartner@ucsf.edu.
5
Chan Zuckerberg Biohub, San Francisco, CA 94158.
6
Department of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences, University of California, San Francisco, CA 94158; arabate@gmail.com zev.gartner@ucsf.edu.

Abstract

Although the elementary unit of biology is the cell, high-throughput methods for the microscale manipulation of cells and reagents are limited. The existing options either are slow, lack single-cell specificity, or use fluid volumes out of scale with those of cells. Here we present printed droplet microfluidics, a technology to dispense picoliter droplets and cells with deterministic control. The core technology is a fluorescence-activated droplet sorter coupled to a specialized substrate that together act as a picoliter droplet and single-cell printer, enabling high-throughput generation of intricate arrays of droplets, cells, and microparticles. Printed droplet microfluidics provides a programmable and robust technology to construct arrays of defined cell and reagent combinations and to integrate multiple measurement modalities together in a single assay.

KEYWORDS:

cell printing; droplet array; droplet microfluidics; fluorescence-activated droplet sorting; single-cell analysis

PMID:
28760972
PMCID:
PMC5565430
DOI:
10.1073/pnas.1704020114
[Indexed for MEDLINE]
Free PMC Article

Conflict of interest statement

Conflict of interest statement: R.H.C., Z.J.G., and A.R.A. are shareholders in Scribe Biosciences, Inc., a company incorporated to commercialize the printed droplet microfluidics technology described in this paper.

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