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Nat Methods. 2017 Sep;14(9):865-868. doi: 10.1038/nmeth.4380. Epub 2017 Jul 31.

Simultaneous epitope and transcriptome measurement in single cells.

Author information

1
New York Genome Center, New York, New York, USA.
2
New York University Medical Center, New York, New York, USA.
3
New York University Center for Genomics and Systems Biology, New York, New York, USA.

Abstract

High-throughput single-cell RNA sequencing has transformed our understanding of complex cell populations, but it does not provide phenotypic information such as cell-surface protein levels. Here, we describe cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq), a method in which oligonucleotide-labeled antibodies are used to integrate cellular protein and transcriptome measurements into an efficient, single-cell readout. CITE-seq is compatible with existing single-cell sequencing approaches and scales readily with throughput increases.

PMID:
28759029
PMCID:
PMC5669064
DOI:
10.1038/nmeth.4380
[Indexed for MEDLINE]
Free PMC Article

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