Format

Send to

Choose Destination
Int J Mol Sci. 2017 Jul 31;18(8). pii: E1634. doi: 10.3390/ijms18081634.

Transcriptome Analysis Reveals Increases in Visceral Lipogenesis and Storage and Activation of the Antigen Processing and Presentation Pathway during the Mouth-Opening Stage in Zebrafish Larvae.

Xu H1,2,3, Liu E4,5, Li Y6,7,8, Li X9,10, Ding C11,12.

Author information

1
College of Animal Science and Technology, Southwest University, Chongqing 400715, China. Xuhao@email.swu.edu.cn.
2
The Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Southwest University, Chongqing 400715, China. Xuhao@email.swu.edu.cn.
3
Institute of Three Gorges Ecological Fisheries of Chongqing, Chongqing 400715, China. Xuhao@email.swu.edu.cn.
4
College of Animal Science and Technology, Southwest University, Chongqing 400715, China. liuenxiu93@email.swu.edu.cn.
5
Institute of Three Gorges Ecological Fisheries of Chongqing, Chongqing 400715, China. liuenxiu93@email.swu.edu.cn.
6
College of Animal Science and Technology, Southwest University, Chongqing 400715, China. aquatics@swu.edu.cn.
7
The Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Southwest University, Chongqing 400715, China. aquatics@swu.edu.cn.
8
Institute of Three Gorges Ecological Fisheries of Chongqing, Chongqing 400715, China. aquatics@swu.edu.cn.
9
College of Animal Science and Technology, Southwest University, Chongqing 400715, China. lxj0125@email.swu.edu.cn.
10
Institute of Three Gorges Ecological Fisheries of Chongqing, Chongqing 400715, China. lxj0125@email.swu.edu.cn.
11
College of Animal Science and Technology, Southwest University, Chongqing 400715, China. dcy866qq@emial.swu.edu.cn.
12
Institute of Three Gorges Ecological Fisheries of Chongqing, Chongqing 400715, China. dcy866qq@emial.swu.edu.cn.

Abstract

The larval phase of the fish life cycle has the highest mortality, particularly during the transition from endogenous to exogenous feeding. However, the transcriptional events underlying these processes have not been fully characterized. To understand the molecular mechanisms underlying mouth-opening acclimation, RNA-seq was used to investigate the transcriptional profiles of the endogenous feeding, mixed feeding and exogenous feeding stages of zebrafish larvae. Differential expression analysis showed 2172 up-regulated and 2313 down-regulated genes during this stage. Genes associated with the assimilation of exogenous nutrients such as the arachidonic acid metabolism, linoleic acid metabolism, fat digestion and absorption, and lipogenesis were activated significantly, whereas dissimilation including the cell cycle, homologous recombination, and fatty acid metabolism were inhibited, indicating a physiological switch for energy storage occurred during the mouth-opening stage. Moreover, the immune recognition involved in the antigen processing and presentation pathway was activated and nutritional supply seemed to be required in this event confirmed by qPCR. These results suggested the energy utilization during the mouth-opening stage is more tended to be reserved or used for some important demands, such as activity regulation, immune defense, and lipid deposition, instead of rapid growth. The findings of this study are important for understanding the physiological switches during the mouth-opening stage.

KEYWORDS:

differentially expressed genes; energy storage; mouth-opening stage; transcriptomic analysis; zebrafish larvae

PMID:
28758957
PMCID:
PMC5578024
DOI:
10.3390/ijms18081634
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Multidisciplinary Digital Publishing Institute (MDPI) Icon for PubMed Central
Loading ...
Support Center