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Int J Mol Sci. 2017 Jul 30;18(8). pii: E1659. doi: 10.3390/ijms18081659.

Chemical Constituents from Hericium erinaceus Promote Neuronal Survival and Potentiate Neurite Outgrowth via the TrkA/Erk1/2 Pathway.

Author information

1
Shaanxi Key Labotory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University, Yangling 712100, China. 08cxzk3zc@163.com.
2
Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Tokushima 770-8514, Japan. 08cxzk3zc@163.com.
3
Shaanxi Key Labotory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University, Yangling 712100, China. caocy@nwsuaf.edu.cn.
4
Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Tokushima 770-8514, Japan. miwa-k@ph.bunri-u.ac.jp.
5
Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Tokushima 770-8514, Japan. kenichi@ph.bunri-u.ac.jp.
6
Shaanxi Key Labotory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University, Yangling 712100, China. xty@nwsuaf.edu.cn.
7
Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Tokushima 770-8514, Japan. jinminggao@nwsuaf.edu.cn.
8
Shaanxi Key Labotory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University, Yangling 712100, China. fukuyama@ph.bunri-u.ac.jp.

Abstract

Hericium erinaceus is a culinary-medicinal mushroom used traditionally in Eastern Asia to improve memory. In this work, we investigated the neuroprotective and neuritogenic effects of the secondary metabolites isolated from the MeOH extract of cultured mycelium of H. erinaceus and the primary mechanisms involved. One new dihydropyridine compound (6) and one new natural product (2) together with five known compounds (1,3-5,7) were obtained and their structures were elucidated by spectroscopic analysis, including 2D NMR and HRMS. The cell-based screening for bioactivity showed that 4-chloro-3,5-dimethoxybenzoic methyl ester (1) and a cyathane diterpenoid, erincine A (3), not only potentiated NGF-induced neurite outgrowth but also protected neuronally-differentiated cells against deprivation of NGF in PC12 pheochromocytoma cells. Additionally, compound 3 induced neuritogenesis in primary rat cortex neurons. Furthermore, our results revealed that TrkA-mediated and Erk1/2-dependant pathways could be involved in 1 and 3-promoted NGF-induced neurite outgrowth in PC12 cells.

KEYWORDS:

4-chloro-3,5-dimethoxybenzoic methyl ester; Hericium erinaceus; PC12 cell; cyathane diterpenoid; erinacine A; neuritogenesis

PMID:
28758954
PMCID:
PMC5578049
DOI:
10.3390/ijms18081659
[Indexed for MEDLINE]
Free PMC Article

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