Format

Send to

Choose Destination
New Phytol. 2017 Oct;216(1):124-135. doi: 10.1111/nph.14705. Epub 2017 Jul 31.

High-resolution synchrotron imaging shows that root hairs influence rhizosphere soil structure formation.

Author information

1
Bioengineering Sciences Research Group, Engineering Sciences Academic Unit, Faculty of Engineering and the Environment, University of Southampton, Southampton, SO17 1BJ, UK.
2
Ecological Sciences Group, The James Hutton Institute, Invergowrie, Dundee, DD2 5DA, UK.
3
Institute of Biological and Environmental Science, University of Aberdeen, Aberdeen, AB24 3UU, UK.
4
School of Science and Engineering, University of Dundee, Dundee, DD1 4HN, UK.

Abstract

In this paper, we provide direct evidence of the importance of root hairs on pore structure development at the root-soil interface during the early stage of crop establishment. This was achieved by use of high-resolution (c. 5 μm) synchrotron radiation computed tomography (SRCT) to visualise both the structure of root hairs and the soil pore structure in plant-soil microcosms. Two contrasting genotypes of barley (Hordeum vulgare), with and without root hairs, were grown for 8 d in microcosms packed with sandy loam soil at 1.2 g cm-3 dry bulk density. Root hairs were visualised within air-filled pore spaces, but not in the fine-textured soil regions. We found that the genotype with root hairs significantly altered the porosity and connectivity of the detectable pore space (> 5 μm) in the rhizosphere, as compared with the no-hair mutants. Both genotypes showed decreasing pore space between 0.8 and 0.1 mm from the root surface. Interestingly the root-hair-bearing genotype had a significantly greater soil pore volume-fraction at the root-soil interface. Effects of pore structure on diffusion and permeability were estimated to be functionally insignificant under saturated conditions when simulated using image-based modelling.

KEYWORDS:

Hordeum vulgare ; image-based modelling; noninvasive imaging; rhizosphere; root hairs; soil structure; synchrotron

PMID:
28758681
PMCID:
PMC5601222
DOI:
10.1111/nph.14705
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Wiley Icon for PubMed Central
Loading ...
Support Center