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Int J Mol Sci. 2017 Jul 25;18(8). pii: E1611. doi: 10.3390/ijms18081611.

Expression of Serum Exosomal and Esophageal MicroRNA in Rat Reflux Esophagitis.

Author information

1
Department of Gastroenterology, Osaka City University Graduate School of Medicine, Osaka 545-8585, Japan. uemurarisa@med.osaka-cu.ac.jp.
2
Department of Hepatology, Osaka City University Graduate School of Medicine, Osaka 545-8585, Japan. yoshimurak@med.osaka-cu.ac.jp.
3
Department of Gastroenterology, Osaka City University Graduate School of Medicine, Osaka 545-8585, Japan. m2075060@med.osaka-cu.ac.jp.
4
Department of Gastroenterology, Osaka City University Graduate School of Medicine, Osaka 545-8585, Japan. m1164972@med.osaka-cu.ac.jp.
5
Department of Gastroenterology, Osaka City University Graduate School of Medicine, Osaka 545-8585, Japan. kojiotani@med.osaka-cu.ac.jp.
6
Department of Gastroenterology, Osaka City University Graduate School of Medicine, Osaka 545-8585, Japan. koichit0802@gmail.com.
7
Department of Gastroenterology, Osaka City University Graduate School of Medicine, Osaka 545-8585, Japan. m1265271@med.osaka-cu.ac.jp.
8
Department of Gastroenterology, Osaka City University Graduate School of Medicine, Osaka 545-8585, Japan. yasuaki1975@gmail.com.
9
Department of Gastroenterology, Osaka City University Graduate School of Medicine, Osaka 545-8585, Japan. m2079981@med.osaka-cu.ac.jp.
10
Department of Gastroenterology, Osaka City University Graduate School of Medicine, Osaka 545-8585, Japan. nkamata@med.osaka-cu.ac.jp.
11
Department of Gastroenterology, Osaka City University Graduate School of Medicine, Osaka 545-8585, Japan. yamagami@med.osaka-cu.ac.jp.
12
Department of Gastroenterology, Osaka City University Graduate School of Medicine, Osaka 545-8585, Japan. ttanigawa@med.osaka-cu.ac.jp.
13
Department of Gastroenterology, Osaka City University Graduate School of Medicine, Osaka 545-8585, Japan. watanabet@med.osaka-cu.ac.jp.
14
Department of Physics, Chuo University, Tokyo 112-8551, Japan. tag@granular.com.
15
Department of Gastroenterology, Osaka City University Graduate School of Medicine, Osaka 545-8585, Japan. yasu@med.osaka-cu.ac.jp.

Abstract

Gastroesophageal reflux disease (GERD) is a common upper gastrointestinal disease. However, the role of exosomal microRNAs (miRNAs) and esophageal miRNAs in GERD has not been studied. A rat model of acid reflux esophagitis was used to establish a novel diagnosis marker for GERD and examine dynamics of miRNA expression in GERD. Rats were sacrificed 3 (acute phase), 7 (sub-acute phase) and 21 days (chronic phase) after induction of esophagitis. Exosomes were extracted from serum, and the expression patterns of serum miRNAs were analyzed. Four upregulated miRNAs (miR-29a-3p, 128-3p, 223-3p and 3473) were identified by microarray analysis. The expression levels of exosomal miR-29a-3p were significantly higher in the chronic phase of reflux esophagitis compared with controls, and increased expression of miR-29a-3p was specific to chronic reflux esophagitis. Esophageal miR-223-3p expression was higher compared with controls, and gradually decreased from acute to chronic phase in esophagitis. In conclusion, exosomal miR-29a-3p and esophageal miR-223-3p might play roles in GERD.

KEYWORDS:

exosome; gastroesophageal reflux disease; microRNA; non-erosive reflux disease; reflux esophagitis

PMID:
28757556
PMCID:
PMC5578003
DOI:
10.3390/ijms18081611
[Indexed for MEDLINE]
Free PMC Article

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