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Adv Exp Med Biol. 2017;959:215-230. doi: 10.1007/978-3-319-55780-9_20.

Fah Knockout Animals as Models for Therapeutic Liver Repopulation.

Author information

1
Oregon Stem Cell Center, Department of Pediatrics, Oregon Health and Science University, Portland, OR, 97239-3098, USA. grompem@ohsu.edu.

Abstract

Several animal models of Fah deficiency have been developed, including mice, pigs and most recently rats. Initially, the murine models were developed with the intent to mirror the human disease for pathophysiologic and therapeutic studies. However, it soon became apparent that Fah-positive hepatocytes have a potent selective growth advantage in mutant liver and can extensively repopulate the diseased organ. For this reason, Fah mutant mice have become a workhorse for liver biology and are widely used in liver stem cell and hepatic gene therapy research. Immune deficient Fah-knockout mice can be repopulated with human hepatocytes, creating "mice with human livers". These chimeric animals have become an important preclinical model for infectious diseases, metabolism and gene therapy. The potent expansion of human hepatocytes in Fah knockout mice has given rise to the concept of using Fah mutants as living bioreactors to produce large quantities of fully mature hepatocytes. As a consequence, larger animal models of Fah deficiency have recently been developed.

KEYWORDS:

Animal model; Cell transplantation; Chimeric animals; Gene therapy; Hepatocellular carcinoma; Hepatocyte; Liver repopulation; Selective advantage

PMID:
28755199
DOI:
10.1007/978-3-319-55780-9_20
[Indexed for MEDLINE]

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