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J Neuroinflammation. 2017 Jul 28;14(1):152. doi: 10.1186/s12974-017-0926-2.

Anticoagulation with warfarin and rivaroxaban ameliorates experimental autoimmune encephalomyelitis.

Author information

1
Department of Neurology, Goethe University, Schleusenweg 2-16, 60528, Frankfurt am Main, Germany. l.stolz@em.uni-frankfurt.de.
2
Dr. Senckenbergische Anatomie, Institute for Anatomy II, Goethe University, Frankfurt am Main, Germany.
3
Pharmazentrum Frankfurt, Institute for General Pharmacology and Toxicology, Goethe University, Frankfurt am Main, Germany.
4
Institute of Neurology (Edinger Institute), Goethe University, Frankfurt am Main, Germany.
5
Neurological Clinic Medical Park, Bad Camberg, Germany.
6
Department of Neurology, Goethe University, Schleusenweg 2-16, 60528, Frankfurt am Main, Germany.

Abstract

BACKGROUND:

In multiple sclerosis, coagulation factors have been shown to modulate inflammation. In this translational study, we investigated whether long-term anticoagulation with warfarin or rivaroxaban has beneficial effects on the course of autoimmune experimental encephalomyelitis (EAE).

METHODS:

Female SJL/J mice treated with anticoagulants namely warfarin or rivaroxaban were immunized with PLP139-151. Stable anticoagulation was maintained throughout the entire experiment. Mice without anticoagulation treated with the vehicle only were used as controls. The neurological deficit was recorded during the course of EAE, and histopathological analyses of inflammatory lesions were performed.

RESULTS:

In preventive settings, both treatment with warfarin and rivaroxaban reduced the maximum EAE score as compared to the control group and led to a reduction of inflammatory lesions in the spinal cord. In contrast, therapeutic treatment with warfarin had no beneficial effects on the clinical course of EAE. Signs of intraparenchymal hemorrhage at the site of the inflammatory lesions were not observed.

CONCLUSION:

We developed long-term anticoagulation models that allowed exploring the course of EAE under warfarin and rivaroxaban treatment. We found a mild preventive effect of both warfarin and rivaroxaban on neurological deficits and local inflammation, indicating a modulation of the disease induction by anticoagulation.

KEYWORDS:

Anticoagulation; Experimental autoimmune encephalomyelitis; Inflammation; Mice; Multiple sclerosis

PMID:
28754118
PMCID:
PMC5534067
DOI:
10.1186/s12974-017-0926-2
[Indexed for MEDLINE]
Free PMC Article

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