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Breastfeed Med. 2017 Nov;12(9):566-569. doi: 10.1089/bfm.2017.0051. Epub 2017 Jul 28.

The Impact of Storage Conditions on the Stability of Lactobacillus rhamnosus GG and Bifidobacterium animalis subsp. lactis Bb12 in Human Milk.

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1 Functional Foods Forum, Faculty of Medicine, University of Turku , Turku, Finland .
2 Food Chemistry and Food Development, Department of Biochemistry, University of Turku , Turku, Finland .
3 Division of Medical Microbiology, Department of Pathology, University of Cape Town , Cape Town, South Africa .
4 Department of Pediatrics, University of Turku and Turku University Hospital , Turku, Finland .


Human milk is the optimal source of complete nutrition for neonates and it also guides the development of infant gut microbiota. Importantly, human milk can be supplemented with probiotics to complement the health benefits of breastfeeding. Storage of human milk for limited periods of time is often unavoidable, but little is known about the effect of different storage conditions (temperature) on the viability of the added probiotics. Therefore, in this study, we evaluated how different storage conditions affect the viability of two specific widely used probiotics, Lactobacillus rhamnosus GG (LGG) and Bifidobacterium animalis subsp. lactis (Bb12), in human milk by culturing and quantitative polymerase chain reaction. Our results indicate that LGG and Bb12 remained stable throughout the storage period. Thus, we conclude that human milk offers an appropriate matrix for probiotic supplementation.


cold storage; human milk; probiotic supplementation; viability

[Indexed for MEDLINE]

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