Format

Send to

Choose Destination
Bioimpacts. 2017;7(2):83-89. doi: 10.15171/bi.2017.11. Epub 2017 Jun 20.

Early endocrine disruptors exposure acts on 3T3-L1 differentiation and endocrine activity.

Boudalia S1,2,3,4,5, Belloir C1,2,3, Miller ML1,2,3, Canivenc-Lavier MC1,2,3.

Author information

1
INRA, UMR1324, Centre des Sciences du Goût et de l'Alimentation, F-21000 Dijon, France.
2
CNRS, UMR6265, Centre des Sciences du Goût et de l'Alimentation, F-21000 Dijon, France.
3
Université de Bourgogne, Centre des Sciences du Goût et de l'Alimentation, F-21000 Dijon, France.
4
Département d'Ecologie et Génie de l'Environnement, Université 8 Mai 1945, Guelma, Algérie.
5
Laboratoire de Biologie, Eau et Environnement, Université 8 Mai 1945, Guelma, Algérie.

Abstract

Introduction: Data from last years suggested that early exposure to endocrine disruptors (EDs) can predispose newborns to endocrine dysfunction of adipocytes, obesity, and associated disorders. The implication of EDs at low doses on adipocyte development has been poorly investigated. For instance, vinclozolin (V) is a dicarboximide fungicide widely used in agriculture since the 90's, alone or in mixture with genistein (G), an isoflavonoid from Leguminosae. This study aims to identify the effect of vinclozolin alone or with genistein, on adipose tissue properties using cell culture. Methods: In steroid-free conditions, 3T3-L1 pre-adipocytes were induced to differentiate in the presence of EDs, singularly or in mixtures, for 2 days. DNA and triglyceride (TG) levels were measured on days 0, 2 and 8 of differentiation. Leptin secretion was measured only on the eighth day. Results: We show that low doses of G (25 µM) and V (0.1 µM) inhibit pre-adipocytes differentiation. This inhibition has been represented by a decreasing in DNA content (µg/well) and decreasing in TG accumulation (mg/mL) in 3T3-L1 cells. Nevertheless, V increased the anti-adipogenic properties of G. Conclusion: This study confirms that EDs singularly or in mixtures, introduced during early stages of life, could affect the differentiation and the endocrine activity of adipocytes, and can act as potential factors for obesity.

KEYWORDS:

Adipose tissue; Endocrine disruptors; Genistein; Leptin; Triglyceride; Vinclozolin

Supplemental Content

Full text links

Icon for PubMed Central
Loading ...
Support Center