Format

Send to

Choose Destination
Acta Trop. 2017 Dec;176:150-161. doi: 10.1016/j.actatropica.2017.07.026. Epub 2017 Jul 24.

Leishmaniasis in the major endemic region of Plurinational State of Bolivia: Species identification, phylogeography and drug susceptibility implications.

Author information

1
Departamento de Parasitología, Facultad de Farmacia, Universidad Complutense de Madrid, Plaza Ramón y Cajal s/n, 28040 Madrid, Spain. Electronic address: pablobil15@yahoo.com.
2
Departamento de Parasitología, Facultad de Farmacia, Universidad Complutense de Madrid, Plaza Ramón y Cajal s/n, 28040 Madrid, Spain; Departamento de Farmacia, Facultad de Ciencias de la Salud, Universidad CEU-Cardenal Herrera, Avda. Seminario s/n, 46113 Moncada, Spain. Electronic address: mda_3000@yahoo.es.
3
Instituto de Investigaciones Fármaco Bioquímicas, Facultad de Ciencias Farmacéuticas y Bioquímicas, Universidad Mayor de San Andrés, Avda. Saavedra 2224, Miraflores, La Paz, Bolivia. Electronic address: bioscar@gmail.com.
4
Instituto de Investigaciones Fármaco Bioquímicas, Facultad de Ciencias Farmacéuticas y Bioquímicas, Universidad Mayor de San Andrés, Avda. Saavedra 2224, Miraflores, La Paz, Bolivia. Electronic address: efrain_salamanca@hotmail.com.
5
INLASA, C/Rafael Zubieta no 1889, Miraflores, La Paz, Bolivia. Electronic address: josesantalla@gmail.com.
6
Departamento de Genética, Facultad de Ciencias Biológicas, Universidad Complutense de Madrid C/José Antonio Novais, 12, 28040 Madrid, Spain. Electronic address: cebe8183@ucm.es.
7
Instituto de Investigaciones Fármaco Bioquímicas, Facultad de Ciencias Farmacéuticas y Bioquímicas, Universidad Mayor de San Andrés, Avda. Saavedra 2224, Miraflores, La Paz, Bolivia. Electronic address: eflores5umsa@gmail.com.
8
Departamento de Parasitología, Facultad de Farmacia, Universidad Complutense de Madrid, Plaza Ramón y Cajal s/n, 28040 Madrid, Spain. Electronic address: francisb@ucm.es.

Abstract

The Plurinational State of Bolivia is one of the Latin American countries with the highest prevalence of leishmaniasis, highlighting the lowlands of the Department of La Paz where about 50% of the total cases were reported. The control of the disease can be seriously compromised by the intrinsic variability of the circulating species that may limit the efficacy of treatment while favoring the emergence of resistance. Fifty-five isolates of Leishmania from cutaneous and mucocutaneous lesions from patients living in different provinces of the Department of La Paz were tested. Molecular characterization of isolates was carried out by 3 classical markers: the rRNA internal transcribed spacer 1 (ITS-1), the heat shock protein 70 (HSP70) and the mitochondrial cytochrome b (Cyt-b). These markers were amplified by PCR and their products digested by the restriction endonuclease enzymes AseI and HaeIII followed by subsequent sequencing of Cyt-b gene and ITS-1 region for subsequent phylogenetic analysis. The combined use of these 3 markers allowed us to assign 36 isolates (65.5%) to the complex Leishmania (Viannia) braziliensis, 4 isolates (7, 27%) to L. (Viannia) lainsoni. and the remaining 15 isolates (23.7%) to a local variant of L. (Leishmania) mexicana. Concerning in vitro drug susceptibility the amastigotes from all isolates where highly sensitive to Fungizone® (mean IC50 between 0.23 and 0.5μg/mL) whereas against Glucantime® the sensitivity was moderate (mean IC50 ranging from 50.84μg/mL for L. (V.) braziliensis to 18.23μg/mL for L. (L.) mexicana. L. (V.) lainsoni was not sensitive to Glucantime®. The susceptibility to miltefosine was highly variable among species isolates, being L. (L.) mexicana the most sensitive, followed by L. (V.) braziliensis and L. (V.) lainsoni (mean IC50 of 8.24μg/mL, 17.85μg/mL and 23.28μg/mL, respectively).

KEYWORDS:

Bolivian isolates; Cytochrome b; Drug susceptibility; HSP-70; ITS-1; Leishmania; Molecular characterization

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center