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Comp Immunol Microbiol Infect Dis. 2017 Aug;53:19-25. doi: 10.1016/j.cimid.2017.06.004. Epub 2017 Jun 23.

Microscopic and molecular identification of hemotropic mycoplasmas in South American coatis (Nasua nasua).

Author information

1
Department of Cell and Molecular Biology, Universidade Federal do Parana, Av. Cel. Francisco H. dos Santos, s/n., Curitiba, PR 81531-980, Brazil; Bela Vista Biological Sanctuary, Itaipu Binacional, R. Teresina, 62, Foz do Iguacu, PR 85866-900, Brazil. Electronic address: mperez@itaipu.gov.br.
2
Department of Veterinary Medicine, Universidade Federal do Parana, R. dos Funcionarios, 1540, Curitiba, PR 80035-050, Brazil.
3
Bela Vista Biological Sanctuary, Itaipu Binacional, R. Teresina, 62, Foz do Iguacu, PR 85866-900, Brazil; Department of Veterinary Medicine, Universidade Federal do Parana, R. dos Funcionarios, 1540, Curitiba, PR 80035-050, Brazil.
4
Bela Vista Biological Sanctuary, Itaipu Binacional, R. Teresina, 62, Foz do Iguacu, PR 85866-900, Brazil.
5
IDEXX Laboratories Inc., 2825 KOVR Drive, West Sacramento, CA 95605, USA.
6
Department of Medicine & Epidemiology, School of Veterinary Medicine, University of California, 2108 Tupper Hall, Davis, CA 95616, USA.
7
Department of Cell and Molecular Biology, Universidade Federal do Parana, Av. Cel. Francisco H. dos Santos, s/n., Curitiba, PR 81531-980, Brazil.
8
Department of Cell and Molecular Biology, Universidade Federal do Parana, Av. Cel. Francisco H. dos Santos, s/n., Curitiba, PR 81531-980, Brazil; Department of Veterinary Medicine, Universidade Federal do Parana, R. dos Funcionarios, 1540, Curitiba, PR 80035-050, Brazil.

Abstract

Hemoplasmas were detected in two apparently healthy captive South American coatis (Nasua nasua) from southern Brazil during an investigation for vector-borne pathogens. Blood was subjected to packed cell volume (PCV) determination, a commercial real-time PCR panel for the detection of Anaplasma spp., Babesia spp., Bartonella spp., Hepatozoon spp., Leishmania spp., Mycoplasma haemofelis, 'Candidatus Mycoplasma turicensis', 'Candidatus Mycoplasma haemominutum', Neorickettsia risticii, Rickettsia rickettsii and Leptospira spp., and a pan-hemoplasma conventional PCR assay. PCV was normal, but both coatis tested positive for hemoplasmas and negative for all the remaining pathogens tested. Using different techniques for microscopy (light, confocal or SEM), structures compatible with hemoplasmas were identified. Sequencing of the 16S rRNA gene identified an organism resembling Mycoplasma haemofelis and another hemotropic Mycoplasma sp., with a sequence identity of 96.8% to a Mycoplasma sp. previously detected in capybaras.

KEYWORDS:

Hemoplasma; Mycoplasma haemofelis; Vector-borne disease; Wild mammal

PMID:
28750863
DOI:
10.1016/j.cimid.2017.06.004
[Indexed for MEDLINE]

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