Format

Send to

Choose Destination
Water Res. 2017 Nov 1;124:77-84. doi: 10.1016/j.watres.2017.07.050. Epub 2017 Jul 20.

Operation-driven heterogeneity and overlooked feed-associated populations in global anaerobic digester microbiome.

Author information

1
Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
2
Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, USA; Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan.
3
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan.
4
Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, USA; Department of Environmental Systems Engineering, Nagaoka University of Technology, Kami-tomioka, Niigata, Japan.
5
Section Sanitary Engineering, Department of Water Management, Delft University of Technology, Delft, The Netherlands.
6
Department of Civil and Environmental Engineering, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
7
School of the Environment, Nanjing University, Nanjing, Jiangsu, China.
8
School of Energy and Environment, City University of Hong Kong, Kowloon, Hong Kong.
9
Department of Botany and Microbiology, University of Oklahoma, Norman, OK, USA.
10
Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, USA. Electronic address: wtliu@illinois.edu.

Abstract

Anaerobic digester (AD) microbiomes harbor complex, interacting microbial populations to achieve biomass reduction and biogas production, however how they are influenced by operating conditions and feed sludge microorganisms remain unclear. These were addressed by analyzing the microbial communities of 90 full-scale digesters at 51 municipal wastewater treatment plants from five countries. Heterogeneity detected in community structures suggested that no single AD microbiome could be defined. Instead, the AD microbiomes were classified into eight clusters driven by operating conditions (e.g., pretreatment, temperature range, and salinity), whereas geographic location of the digesters did not have significant impacts. Comparing digesters populations with those present in the corresponding feed sludge led to the identification of a hitherto overlooked feed-associated microbial group (i.e., the residue populations). They accounted for up to 21.4% of total sequences in ADs operated at low temperature, presumably due to ineffective digestion, and as low as 0.8% in ADs with pretreatment. Within each cluster, a core microbiome was defined, including methanogens, syntrophic metabolizers, fermenters, and the newly described residue populations. Our work provides insights into the key factors shaping full-scale AD microbiomes in a global scale, and draws attentions to the overlooked residue populations.

KEYWORDS:

Anaerobic digester; Feed sludge; Microbiome; Operation

PMID:
28750287
DOI:
10.1016/j.watres.2017.07.050
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center