Send to

Choose Destination
J Anim Sci Technol. 2017 Jul 24;59:16. doi: 10.1186/s40781-017-0141-9. eCollection 2017.

Role of ghrelin in the pancreatic exocrine secretion via mitogen-activated protein kinase signaling in rats.

Lee KH#1,2, Lee JS#1,3, Wang T4, Oh JJ5, Roh S6, Lee HG1,3.

Author information

Department of Animal Science and Technology, College of Animal Bioscience and Technology, Konkuk University, Seoul, 05029 South Korea.
Research Department, Korea Industrial Co., Ltd., Pusan, 46978 South Korea.
Team of Educational Program for Specialists in Global Animal Science, Brain Korea 21 Plus Project, Konkuk University, Seoul, 05029 South Korea.
College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118 China.
Natural Product Clinical Research Center, Clinical Research Center, Pusan National University School of Medicine, Busan, 49241 South Korea.
Laboratory of Animal Physiology, Graduate School of Agricultural Science, Tohoku University, Sendai, 981-8555 Japan.
Contributed equally



This study was performed to investigate the impact of exogenous ghrelin on the pancreatic α-amylase outputs and responses of pancreatic proteins to ghrelin that may relate to pancreatic exocrine.


Sprague-Dawley male rats (9 weeks old, 300 ± 10 g) were injected with ghrelin via intraperitoneal (i.p.) infusion at dosage of 0, 0.1, 1.0 and 10.0 μg/kg body weight (BW), respectively. The plasma ghrelin and cholecystokinin (CCK) level were determined using enzyme immunoassay kit; the mRNA expression of ghrelin receptor (GHSR-1α) and growth hormone (GH) receptor were assessed by reverse transcription PCR; the expressions of pancreatic α-amylase activity, extracellular-signal-regulated kinases (ERK), phosphorylated extracellular-signal-regulated kinases (pERK) and c-Jun N-terminal kinase (JNK) were evaluated by western blotting; moreover the responses of pancreatic proteins to ghrelin were analyzed using the two-dimensional gel electrophoresis system.


The exogenous ghrelin (1.0 and 10.0 μg/kg BW) elevated the level of plasma ghrelin (p < 0.05), and suppressed the expression of pancreatic α-amylase at a dose of 10.0 μg/kg BW (p < 0.05). No difference in the level of plasma CCK was observed, even though rats were exposed to any dose of exogenous ghrelin. In addition, a combination of western blot and proteomic analysis revealed exogenous ghrelin (10.0 μg/kg BW) induced increasing the JNK and ERK expressions (p < 0.05) and four proteins such as Destrin, Anionic trypsin-1, Trypsinogen, and especially eukaryotic translation initiation factor 3 in rat pancreas.


Taken together, exogenous ghrelin by i.p. infusion plays a role in the pancreatic exocrine secretion via mitogen-activated protein kinase signaling pathway.


Cholecystokinin; Ghrelin; Pancreatic exocrine; Sprague-Dawley rats; α-Amylase activity

Supplemental Content

Full text links

Icon for BioMed Central Icon for PubMed Central
Loading ...
Support Center