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J Anim Sci Technol. 2017 Jul 24;59:16. doi: 10.1186/s40781-017-0141-9. eCollection 2017.

Role of ghrelin in the pancreatic exocrine secretion via mitogen-activated protein kinase signaling in rats.

Lee KH#1,2, Lee JS#1,3, Wang T4, Oh JJ5, Roh S6, Lee HG1,3.

Author information

1
Department of Animal Science and Technology, College of Animal Bioscience and Technology, Konkuk University, Seoul, 05029 South Korea.
2
Research Department, Korea Industrial Co., Ltd., Pusan, 46978 South Korea.
3
Team of Educational Program for Specialists in Global Animal Science, Brain Korea 21 Plus Project, Konkuk University, Seoul, 05029 South Korea.
4
College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118 China.
5
Natural Product Clinical Research Center, Clinical Research Center, Pusan National University School of Medicine, Busan, 49241 South Korea.
6
Laboratory of Animal Physiology, Graduate School of Agricultural Science, Tohoku University, Sendai, 981-8555 Japan.
#
Contributed equally

Abstract

BACKGROUND:

This study was performed to investigate the impact of exogenous ghrelin on the pancreatic α-amylase outputs and responses of pancreatic proteins to ghrelin that may relate to pancreatic exocrine.

METHODS:

Sprague-Dawley male rats (9 weeks old, 300 ± 10 g) were injected with ghrelin via intraperitoneal (i.p.) infusion at dosage of 0, 0.1, 1.0 and 10.0 μg/kg body weight (BW), respectively. The plasma ghrelin and cholecystokinin (CCK) level were determined using enzyme immunoassay kit; the mRNA expression of ghrelin receptor (GHSR-1α) and growth hormone (GH) receptor were assessed by reverse transcription PCR; the expressions of pancreatic α-amylase activity, extracellular-signal-regulated kinases (ERK), phosphorylated extracellular-signal-regulated kinases (pERK) and c-Jun N-terminal kinase (JNK) were evaluated by western blotting; moreover the responses of pancreatic proteins to ghrelin were analyzed using the two-dimensional gel electrophoresis system.

RESULTS:

The exogenous ghrelin (1.0 and 10.0 μg/kg BW) elevated the level of plasma ghrelin (p < 0.05), and suppressed the expression of pancreatic α-amylase at a dose of 10.0 μg/kg BW (p < 0.05). No difference in the level of plasma CCK was observed, even though rats were exposed to any dose of exogenous ghrelin. In addition, a combination of western blot and proteomic analysis revealed exogenous ghrelin (10.0 μg/kg BW) induced increasing the JNK and ERK expressions (p < 0.05) and four proteins such as Destrin, Anionic trypsin-1, Trypsinogen, and especially eukaryotic translation initiation factor 3 in rat pancreas.

CONCLUSIONS:

Taken together, exogenous ghrelin by i.p. infusion plays a role in the pancreatic exocrine secretion via mitogen-activated protein kinase signaling pathway.

KEYWORDS:

Cholecystokinin; Ghrelin; Pancreatic exocrine; Sprague-Dawley rats; α-Amylase activity

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