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J Dent Res Dent Clin Dent Prospects. 2017 Spring;11(2):73-77. doi: 10.15171/joddd.2017.014. Epub 2017 Jun 21.

Comparison of the effect of three autogenous bone harvesting methods on cell viability in rabbits.

Author information

1
Department of Periodontics, Faculty of Dentistry, Hamadan University of Medical Sciences, Hamadan, Iran.
2
Department of Anatomical Science, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
3
Department of Immunology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
4
Modeling of Noncommunicable Diseases Research Center, Department of Biostatistics, Faculty of Public Health, Hamadan University of Medical Sciences, Hamadan, Iran.

Abstract

Background. This study was designed to compare the viability of autogenous bone grafts, harvested using different methods, in order to determine the best harvesting technique with respect to more viable cells. Methods. In this animal experimental study, three harvesting methods, including manual instrument (chisel), rotary device and piezosurgery, were used for harvesting bone grafts from the lateral body of the mandible on the left and right sides of 10 rabbits. In each group, 20 bone samples were collected and their viability was assessed using MTS kit. Statistical analyses, including ANOVA and post hoc Tukey tests, were used for evaluating significant differences between the groups. Results. One-way ANOVA showed significant differences between all the groups (P=0.000). Data analysis using post hoc Tukey tests indicated that manual instrument and piezosurgery had no significant differences with regard to cell viability (P=0.749) and the cell viability in both groups was higher than that with the use of a rotary instrument (P=0.000). Conclusion. Autogenous bone grafts harvested with a manual instrument and piezosurgery had more viable cells in comparison to the bone chips harvested with a rotary device.

KEYWORDS:

Autograft; autologous transplants; dental Instruments; piezosurgery; tissue harvesting

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