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Endocr Relat Cancer. 2017 Oct;24(10):531-541. doi: 10.1530/ERC-17-0117. Epub 2017 Jul 26.

Molecular characteristics of the KCNJ5 mutated aldosterone-producing adenomas.

Author information

1
Department of Molecular Endocrinology and MetabolismGraduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
2
Department of Molecular Endocrinology and MetabolismGraduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan tyoshimoto.mem@tmd.ac.jp.
3
Department of Maternal-Fetal BiologyNational Research Institute for Child Health and Development, Tokyo, Japan.
4
Department of Systems BioMedicineNational Research Institute for Child Health and Development, Tokyo, Japan.
5
Department of UrologyGraduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
6
Department of Preemptive Medicine and MetabolismGraduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
7
Department of Medical and Bioregulatory ScienceGraduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
8
Japan Science and Technology AgencyCREST, AMED, Tokyo, Japan.

Abstract

The pathophysiology of aldosterone-producing adenomas (APAs) has been investigated via genetic approaches and the pathogenic significance of a series of somatic mutations, including KCNJ5, has been uncovered. However, how the mutational status of an APA is associated with its molecular characteristics, including its transcriptome and methylome, has not been fully understood. This study was undertaken to explore the molecular characteristics of APAs, specifically focusing on APAs with KCNJ5 mutations as opposed to those without KCNJ5 mutations, by comparing their transcriptome and methylome status. Cortisol-producing adenomas (CPAs) were used as reference. We conducted transcriptome and methylome analyses of 29 APAs with KCNJ5 mutations, 8 APAs without KCNJ5 mutations and 5 CPAs. Genome-wide gene expression and CpG methylation profiles were obtained from RNA and DNA samples extracted from these 42 adrenal tumors. Cluster analysis of the transcriptome and methylome revealed molecular heterogeneity in APAs depending on their mutational status. DNA hypomethylation and gene expression changes in Wnt signaling and inflammatory response pathways were characteristic of APAs with KCNJ5 mutations. Comparisons between transcriptome data from our APAs and that from normal adrenal cortex obtained from the Gene Expression Omnibus suggested similarities between APAs with KCNJ5 mutations and zona glomerulosa. The present study, which is based on transcriptome and methylome analyses, indicates the molecular heterogeneity of APAs depends on their mutational status. Here, we report the unique characteristics of APAs with KCNJ5 mutations.

KEYWORDS:

adenoma; adrenal cortex; gene expression; molecular genetics

PMID:
28747387
DOI:
10.1530/ERC-17-0117
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