Abstract
The effects of somatostatin and dopaminergic agents on pituitary cyclic nucleotide phosphodiesterases that had been partially purified through DEAE cellulose column chromatography were studied. Somatostatin, L-dopa, dopamine and CB-154(2-bromo-alpha-ergocriptine) competitively inhibited the pituitary cyclic AMP phosphodiesterase activity, especially that of the low Km enzyme. This inhibition was most potent in the case of somatostatin and CB-154.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3',5'-Cyclic-AMP Phosphodiesterases / isolation & purification
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3',5'-Cyclic-AMP Phosphodiesterases / metabolism*
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3',5'-Cyclic-GMP Phosphodiesterases / isolation & purification
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3',5'-Cyclic-GMP Phosphodiesterases / metabolism*
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Animals
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Bromocriptine / pharmacology*
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Cattle
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Dopamine / pharmacology*
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Kinetics
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Levodopa / pharmacology*
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Pituitary Gland, Anterior / enzymology*
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Pituitary Gland, Posterior / enzymology*
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Somatostatin / pharmacology*
Substances
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Bromocriptine
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Levodopa
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Somatostatin
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3',5'-Cyclic-AMP Phosphodiesterases
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3',5'-Cyclic-GMP Phosphodiesterases
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Dopamine