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Int J Reprod Biomed (Yazd). 2017 May;15(5):279-286.

Evaluation of intracellular anion superoxide level, heat shock protein A2 and protamine positive spermatozoa percentages in teratoasthenozoospermia.

Author information

1
Department of Anatomy, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.
2
Department Of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
3
Department of Urology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
4
Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

Abstract

BACKGROUND:

Teratoasthenozoospermia (TA) is a severe form of male infertility with no clear etiology.

OBJECTIVE:

To compare the level of intracellular anion superoxide (O2-), heat shock protein A2 (HSPA2) and protamine deficiencies in ejaculated spermatozoa between teratoasthenozoospermic and normozoospermic men.

MATERIALS AND METHODS:

In this case- control study, semen samples of 20 infertile men, with TA (with normal morphology lower than 4%_ and total motility lower than 40% ) as the case group and 20 normozoospermic fertile men as the control group were evaluated for intracellular O2- and HSPA2 by flow cytometry and protamine deficiency by Chromomycin A3 (CMA3) test.

RESULTS:

The rate of CMA3+ spermatozoa in the case group was higher than controls (p=0.001). The percentages of HSPA2+ spermatozoa in the cases were significantly lower than controls (p=0.001). Also, intracellular O2- levels in the case group were significantly higher than controls (p=0.001) and had positive correlations with sperm apoptosis (r=0.79, p=0.01) and CMA3 positive sperm (r=0.76, p=0.01), but negative correlations with normal morphology (r=-0.81, p=0.01) and motility (r=-0.81, p=0.01). There was no significant correlation between intracellular O2- and HSPA2 in the case group (r=0.041, p=0.79).

CONCLUSION:

We suggest that the increase in intracellular O2-, decrease in spermatozoa HSPA2+, and high percentages of spermatozoa with immature chromatin might be considered as etiologies of infertility in TA patients.

KEYWORDS:

HSPA2; Male infertility; Protamine deficiency; Sperm chromatin

PMID:
28744523
PMCID:
PMC5510581

Conflict of interest statement

There is no conflict of interest in this article.

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