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3 Biotech. 2017 Aug;7(4):260. doi: 10.1007/s13205-017-0892-5. Epub 2017 Jul 24.

A sequential pretreatment of lignocelluloses in bamboo biomass to fermentable sugars by acid/enzymatic hydrolysis.

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Department of Chemical Engineering, SRM University, Chennai, Tamilnadu 603203 India.
Department of Industrial Biotechnology, Government College of Technology, Coimbatore, Tamilnadu 641013 India.


A sequential pretreatment method for hydrolyzing rigid hemicelluloses and cellulose content in the bamboo biomass was investigated in this study. The effects of different parameters, such as nature of biomass, type of acid, acid and biomass concentration, were studied. Under the optimum condition of 5% (v/v) HCl-treated biomass and biomass concentration (8%, w/v), the maximum yield of sugar (619 mg/g of biomass) was obtained. The enzymatic hydrolysis parameter conditions were further optimized by response surface methodology-based central composite method. According to the results, the highest yield of sugar (515 mg/g of biomass) was obtained at hydrolysis temperature 50 °C, biomass concentration 8.9%, w/v, enzyme concentration (199.8 mg/g of biomass) and time 60 h, respectively. The effects of untreated, pretreated and enzymatically hydrolyzed biomass structure and complexity were investigated by field emission scanning electron microscopy and X-ray diffraction techniques.


Bamboo; Cellulose; Enzymatic hydrolysis; Optimization; Sequential pretreatment

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