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G3 (Bethesda). 2017 Sep 7;7(9):3177-3184. doi: 10.1534/g3.117.300069.

The Stress-Inducible Peroxidase TSA2 Underlies a Conditionally Beneficial Chromosomal Duplication in Saccharomyces cerevisiae.

Author information

1
Molecular and Computational Biology Section, Department of Biological Sciences, University of Southern California, Los Angeles, California 90089-2910 linderr@uci.edu ian.ehrenreich@usc.edu.
2
Department of Ecology and Evolutionary Biology, School of Biological Sciences, University of California, Irvine, California 92697-2525.
3
Molecular and Computational Biology Section, Department of Biological Sciences, University of Southern California, Los Angeles, California 90089-2910.

Abstract

Although chromosomal duplications are often deleterious, in some cases they enhance cells' abilities to tolerate specific genetic or environmental challenges. Identifying the genes that confer these conditionally beneficial effects to particular chromosomal duplications can improve our understanding of the genetic and molecular mechanisms that enable certain aneuploidies to persist in cell populations and contribute to disease and evolution. Here, we perform a screen for spontaneous mutations that improve the tolerance of haploid Saccharomyces cerevisiae to hydrogen peroxide. Chromosome IV duplication is the most frequent mutation, as well as the only change in chromosomal copy number seen in the screen. Using a genetic mapping strategy that involves systematically deleting segments of a duplicated chromosome, we show that the chromosome IV's duplication effect is largely due to the generation of a second copy of the stress-inducible cytoplasmic thioredoxin peroxidase TSA2 Our findings add to a growing body of literature that shows the conditionally beneficial effects of chromosomal duplication are typically mediated by a small number of genes that enhance tolerance to specific stresses when their copy numbers are increased.

KEYWORDS:

aneuploidy chromosomal; duplication natural genetic variation oxidative stress yeast

PMID:
28743806
PMCID:
PMC5592942
DOI:
10.1534/g3.117.300069
[Indexed for MEDLINE]
Free PMC Article

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