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Development. 2017 Sep 1;144(17):3054-3065. doi: 10.1242/dev.150342. Epub 2017 Jul 25.

Sonic hedgehog from both nerves and epithelium is a key trophic factor for taste bud maintenance.

Author information

1
Department of Cell and Developmental Biology and the Rocky Mountain Taste and Smell Center University of Colorado, Anschutz Medical Campus, Aurora, CO 80045, USA.
2
Graduate Program in Cell Biology, Stem Cells and Development, University of Colorado, Anschutz Medical Campus, Aurora, CO 80045, USA.
3
Neuroscience Graduate Program, University of Colorado, Anschutz Medical Campus, Aurora, CO 80045, USA.
4
Department of Cell and Developmental Biology and the Rocky Mountain Taste and Smell Center University of Colorado, Anschutz Medical Campus, Aurora, CO 80045, USA linda.barlow@ucdenver.edu.

Abstract

The integrity of taste buds is intimately dependent on an intact gustatory innervation, yet the molecular nature of this dependency is unknown. Here, we show that differentiation of new taste bud cells, but not progenitor proliferation, is interrupted in mice treated with a hedgehog (Hh) pathway inhibitor (HPI), and that gustatory nerves are a source of sonic hedgehog (Shh) for taste bud renewal. Additionally, epithelial taste precursor cells express Shh transiently, and provide a local supply of Hh ligand that supports taste cell renewal. Taste buds are minimally affected when Shh is lost from either tissue source. However, when both the epithelial and neural supply of Shh are removed, taste buds largely disappear. We conclude Shh supplied by taste nerves and local taste epithelium act in concert to support continued taste bud differentiation. However, although neurally derived Shh is in part responsible for the dependence of taste cell renewal on gustatory innervation, neurotrophic support of taste buds likely involves a complex set of factors.

KEYWORDS:

Cell renewal; Innervation; Lingual epithelium; Shh; Taste receptor cells

PMID:
28743797
PMCID:
PMC5611957
DOI:
10.1242/dev.150342
[Indexed for MEDLINE]
Free PMC Article

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