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Mol Syst Biol. 2017 Jul 25;13(7):936. doi: 10.15252/msb.20167412.

Capturing protein communities by structural proteomics in a thermophilic eukaryote.

Author information

1
European Molecular Biology Laboratory, Structural and Computational Biology Unit, Heidelberg, Germany.
2
Chair of Bioanalytics, Institute of Biotechnology, Technische Universität Berlin, Berlin, Germany.
3
Cell Networks, Bioquant & Biochemie Zentrum Heidelberg, Heidelberg University, Heidelberg, Germany.
4
Department of Anatomy and Cell Biology, McGill University, Montreal, QC, Canada.
5
Wellcome Trust Centre for Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh, UK.
6
European Molecular Biology Laboratory, Structural and Computational Biology Unit, Heidelberg, Germany martin.beck@embl.de gavin@embl.de.

Abstract

The arrangement of proteins into complexes is a key organizational principle for many cellular functions. Although the topology of many complexes has been systematically analyzed in isolation, their molecular sociology in situ remains elusive. Here, we show that crude cellular extracts of a eukaryotic thermophile, Chaetomium thermophilum, retain basic principles of cellular organization. Using a structural proteomics approach, we simultaneously characterized the abundance, interactions, and structure of a third of the C. thermophilum proteome within these extracts. We identified 27 distinct protein communities that include 108 interconnected complexes, which dynamically associate with each other and functionally benefit from being in close proximity in the cell. Furthermore, we investigated the structure of fatty acid synthase within these extracts by cryoEM and this revealed multiple, flexible states of the enzyme in adaptation to its association with other complexes, thus exemplifying the need for in situ studies. As the components of the captured protein communities are known-at both the protein and complex levels-this study constitutes another step forward toward a molecular understanding of subcellular organization.

KEYWORDS:

computational modeling; cryo‐electron microscopy; fatty acid synthase; interaction proteomics; metabolon

PMID:
28743795
PMCID:
PMC5527848
DOI:
10.15252/msb.20167412
[Indexed for MEDLINE]
Free PMC Article

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