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Genes Dev. 2017 Jun 15;31(12):1272-1287. doi: 10.1101/gad.299347.117. Epub 2017 Jul 25.

Ectopic application of the repressive histone modification H3K9me2 establishes post-zygotic reproductive isolation in Arabidopsis thaliana.

Author information

1
Department of Plant Biology, Uppsala BioCenter, Swedish University of Agricultural Sciences, Linnean Center of Plant Biology, Uppsala 75007, Sweden.
2
Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent 9052, Belgium.
3
VIB Center for Plant Systems Biology, Ghent 9052, Belgium.
4
Department of Biochemistry, Ghent University, Ghent 9052, Belgium.
5
VIB Center for Medical Biotechnology, Ghent 9052, Belgium.

Abstract

Hybrid seed lethality as a consequence of interspecies or interploidy hybridizations is a major mechanism of reproductive isolation in plants. This mechanism is manifested in the endosperm, a dosage-sensitive tissue supporting embryo growth. Deregulated expression of imprinted genes such as ADMETOS (ADM) underpin the interploidy hybridization barrier in Arabidopsis thaliana; however, the mechanisms of their action remained unknown. In this study, we show that ADM interacts with the AT hook domain protein AHL10 and the SET domain-containing SU(VAR)3-9 homolog SUVH9 and ectopically recruits the heterochromatic mark H3K9me2 to AT-rich transposable elements (TEs), causing deregulated expression of neighboring genes. Several hybrid incompatibility genes identified in Drosophila encode for dosage-sensitive heterochromatin-interacting proteins, which has led to the suggestion that hybrid incompatibilities evolve as a consequence of interspecies divergence of selfish DNA elements and their regulation. Our data show that imbalance of dosage-sensitive chromatin regulators underpins the barrier to interploidy hybridization in Arabidopsis, suggesting that reproductive isolation as a consequence of epigenetic regulation of TEs is a conserved feature in animals and plants.

KEYWORDS:

endosperm; heterochromatin; hybrid incompatibility; imprinted genes; polyploidy; transposable elements

PMID:
28743695
PMCID:
PMC5558928
DOI:
10.1101/gad.299347.117
[Indexed for MEDLINE]
Free PMC Article

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